Trypan blue exclusion test

Count the cells by trypan blue exclusion test in a Biirker-Tiirk cell counting chamber and calculate the correct dilution (see below). 

Report cell population viabilities as measured by the Trypan blue exclusion test in the original and sorted cell samples. 

Remove 1 ml of cell suspension into a disposable culture tube for a cell count. Determine the cell concentration of the suspension by direct counting and monitor the viability using the Trypan blue exclusion test. 

Trypan Blue Exclusion Test for Cell Viability... 

For statistical analysis, the greater the number of counts per culture the more accurate will be the statistics. The absolute minimum is triplicate values. Figure 2 shows the selective toxicity of EF13 for HIV-chronically infected cells over uninfected cells established using the Trypan blue exclusion test. 

Figure 35.8 Frequency of trypan-blue (TB) negative cells against the a values before subculture. The a values were evaluated by direct observation according to Eq. (35.1). The TB-stained cells were counted twice by trypan-blue exclusion test among about 100 cells. This figure was reproduced by permission of the Society for Biotechnology, Japan.

Incubation of the hepatocytes, with fructose or glucose, up to 2 h, did not affect the number of hepatocytes, nor their viability, as determined by the trypan blue exclusion test. 

Cell damage can be characterized using a number of techniques, including, but not limited to, trypan blue exclusion test, fluorescein diacetate test. 

SECM inside living cells is a very challenging task. For this, electrode with submicron size tip diameter, special low current potentiostat, and appropriate shielding are needed. The obtained SECM signal is highly valuable from point of view of cell physiology if the measurement causes only minor invasion. It was shown [138] by trypan blue exclusion tests that MCF-lOA breast cells can survive intracellular voltammetric experiments. The test is based on the dye out pumping property of the live cells [139]. That means if trypan blue dye is added (5 pM solution) to the immobilized cells, then after a few hours the dead cells appear blue but live cells appear uncolored. 

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