Cell-based reporter assays characterization

Based mainly on cell-free assays, 10 enzymatic activities that degrade phospholipids, intermediates in the phospholipid biosynthetic pathway, or triacylglycerol have been reported (Table 2). The detergent-resistant phospholipase A, (encoded by pldA) of the outer membrane, characterized by Nojima and colleagues (Y. Nakagawa, 1991), is the most studied of these enzymes. This enzyme is unusually resistant to inactivation by heat and ionic detergents... 

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