Recombinant hybridization

A.faecalis T1 Recombinant hybrid poly(3HB) depol. domain mutants ... 

A second example of a toxin that has been used as targeting device is tetanus toxin. Tetanus toxin is a potent neurotoxin, which can undergo uptake in the nerve endings of motor neurones and subsequent retrograde transport into the central nervous system. The nontoxic C-fragment of tetanus toxin (TTC,451 amino acids), has been used to increase the neuronal uptake of the therapeutic protein SOD [57]. Following intravenous infusion, the recombinant hybrid protein reduced the occurrence of ischaemia-induced cerebral infarction in rats [58]. 

Hybrid and Consensus IFNs. After the sequences of all IFN-a subtypes and their activities were deduced, recombinant hybrids were designed to improve the activity of natural subtypes. It is possible to radically change the properties of an IFN by making hybrids. For example, a hybrid made from the first 61 amino-acid residues of IFN-al and the last 104 amino-acid residues of IFN-a2 exhibited high antiviral activity on mouse cells, while another hybrid produced from the first 91 amino-acid residues of IFN-al and the last 72 amino-acid residues of IFN-a4 had no antiviral effect but displayed enhanced antitumor activity in mice, a property neither of the parent molecules exhibit. Structure-function analysis of hybrid molecules has indicated that the NH2 terminus portion of the IFN-a molecule is important for its biological activity [46]. 

Ohgo, K., Zhao, C.H., Kobayashi, M., and Asakura, T. "Preparation of non-woven nanofibers of Bombyx mori silk, Sarnia cynthia ricini silk and recombinant hybrid silk with electro-spinning method". Polymer 44(3), 841-846 (2003). 

The transgene is often modihed to cnstom design the hnal protein product. Furthermore, many expression vectors are designed to allow various tags to be linked to the expressed recombinant protein. In this manner, the recombinant hybrid protein... 

Infectious substances are those that contain viable micro-organisms that are known or assumed to cause diseases in humans or animals. The viable microorganisms include bacteria, rickettsia, parasites, fungi, and recombined, hybrid, or mutated micro-organisms. 

Class 6.2 Infectious substances. These are substances containing viable micro-organisms including bacterium, virus, rickettsia, parasite, fungus or a recombinant, hybrid or mutant that are known, or reasonably believed, to cause disease in animals or humans. IMO Class 6.2, 1.1.2... 

Pharmacological profiles of the recombinant hybrid insect a/vertebrate yS nAChRs are poorly defined and the binding sites are not established for identified subunits versus native receptors. Functional expression of insect nAChRs of known subunit compositions facilitates understanding of the mechanism underlying these molecular interactions. However, it is difficult to heterologously express functionally robust nAChRs not only in Xenopus oocytes but also in Drosophila S2 cells [186]. Only a few functional receptors have been obtained after expression of different subunit combinations in X. oocytes or cell lines. Nevertheless, Drosophila nAChR a subunits can form homo-oligomeric functional nAChRs when coexpressed with a vertebrate pi (non-a) subunit in X. oocytes. This has been demonstrated so far for ... 

In recent research, recombinant hybrid silk fibers that incorporate both the highly crystalline domains of native silk fibroin from silkworms and the amorphous domains from a different variety of silk (from a wild species of silkworm Samia cynthia ricini) were electrospun fromhexafluoroacetone (HFA) solution... 

Silk/chitosan blends (up to 30% chitosan) Recombinant hybrid silk... 

It is sometimes convenient to combine aos to form hybrid orbitals that have well defined directional character and to then form mos by combining these hybrid orbitals. This recombination of aos to form hybrids is never necessary and never provides any information that could be achieved in its absence. However, forming hybrids often allows one to focus on those interactions among directed orbitals on neighboring atoms that are most important. 

Recombinant DNA (r-DNA) The hybrid DNA produeed by joining pieees of DNA from different organisms together in vitro (i.e., in an artifieial apparatus). 

Verhaegen, M., and Christopoulos, T. K. (2002). Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization. Anal. Chem. 74 4378-4385. 

Fig.l. Gene maps of recombinant plasmids pTOH, pTTOH, pEOH, and pETOH. Abbreviations Pnc, trc promoter Px7, T7 and lac hybrid promoter Tat, twin-arginme TorA signal sequence of TMAO reductase OPH, organophosphoms hydrolase gene Hisg, hexa-histidine affinity tag. 

As for more complicated molecules, the exo-addition 106> in the 2-norbonyl radical was explained from the point of view of SO extension 105>. The 2-carbon is not exactly sp2 hybridized but extends more in the exo direction than in the endo direction15. The nonplanarity of almost-s 2 carbon in radicals is also expected in 2-chloroethyl and 4-t-butylcyclo-hexyl in which stereoselective recombinations are known. A rather exaggerated illustration of the mode of extension of SO MO is given below... 

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