Chitosan blends

Poly-L-lysine-blended chitosan, collagen-blended chitosan and albumin-blended chitosan were also considered, with collagen control material. Culture of PC12 cells and fetal mouse cerebral cortex cells on these biomaterials was used to evaluate their nerve cell affinity. The composite materials, had significantly improved nerve cell affinity compared to chitosan. Poly-L-lysine-blended chitosan exhibited the best nerve cell affinity and was a better material in promoting neurite outgrowth than collagen [328]. 

Svang-Ariyaskul, A., Huang, R.Y.M., Douglas, P.L. et al. 2006. Blended chitosan and polyvinyl alcohol membranes for the pervaporation dehydration of isopropanol,... 

Sarasam, A. R., Krishnaswamy, R. K., and Madihally, S. V. 2006. Blending chitosan with polycaprolactone Effects on physicochemical and antibacterial properties. Biomacromolecules 7 1131-1138. 

Chandy, T. and Sharman, C. P. 1992. Prostaglandin Ej-lmmobilized Poly (vinyl alcohol)-blended chitosan membranes Blood compatibility and permeability properties. J. Appl. Polym. Sci. 44 2145-2156. 

Rahman, M.M., Pervez, S., Nesa, B., Khan, M.A., 2012. Preparation and characterization of porous scaffold composite films by blending chitosan and gelatin solutions for skin tissue engineering. Polym. Int. 62, 79-86. 

Blending chitosan with PLLA suppressed the crystallization of the PLLA component. 

Urokinase AT-III PGEiiMethyl Dopa Complex Immobilized Albumin-Blended Chitosan Membranes - Antithrombotic and Permeability Properties 297... 

UR0RlNASE AT-IlI P6Ei M THYL DOPA COMPLEX IMMOBILIZED ALBUMIN-BLENDED CHITOSAN MEMBRANES - ANTITHROMBOTIC AND PERMEABILITY PROPERTIES... 

Here we report an attempt to immobilize an urokinase antithrombin III PGEirmethyl dopa conjugate on albumin blended chitosan membranes and the evaluation of their antithrombotic and permeability properties. Further studies have also been undertaken to improve the mechanical properties of the albumin blended chitosan membranes through optimum cross-linking with carbodiimide. A novel approach of producing other protein blends of chitosan, as non-thrombogenic membranes, for improved permeability had been demonstrated and compared to that of the standard cellulose membranes. 

The carboxyl groups of the albumin blended chitosan membranes were activated with carbodiimide. The membranes were dipped in a solution containing 1% carbodiimide, in 0.05H phosphate buffer (pH = 8.3) for 30 min. at 4 C, and the excess diimide was washed from the membrane with buffer solution. The complex was dissolved in phosphate buffer (pH = 8.3) and added to the previously equilibrated, activated albumin blended chitosan membranes, and kept 5 hrs. at 4 C for coupling. At the end of this process, the unbound complex was washed off with buffer. These membranes were used for platelet adhesion studies and permeability evaluation. A few albumin chitosan membranes were also exposed to carbodiimide for various time intervals to evaluate the effect of crosslinking on their permeability properties. 

Table 1. The permeability (%) of urea through various albumin blended chitosan membranes (6 hour dialysis).

The results of the platelet adhesion to the urokinase complex modified albumin blended chitosan membranes is shown in Table 3. The number of adhering platelets seen on the urokinase derivative immobilized surface has been dramatically reduced when compared with the albumin blended membrane. The permeability of various molecules through the albumin blended chitosan and the urokinase derivative immobilized mem-branes, as a function of time, is demonstrated in Figures 1 and 2, respectively. It is evident from these studies that the urokinase complex modified membranes had similar permeability properties to those of the... 

Figure 1. Permeability of various compounds through albumin blended chitosan membrane as a fumction of time (from a mixture of compounds). 

Albumin blended chitosan membrane < ACM) A CompJejf immobilized ACM... 

Figure 3. Leakage of the immobilized complex with time, during dialysis, from the complex immobilized albumin blended chitosan membrane, to the permeant solution.

Figure 4. Permeability of urea through various ratios of the albumin blended chitosan membranes (from a mixture of urea, uric acid and creatinine).

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