Recombinant glucocerebrosidase

A. Zimran, D. Elstein, E. Levy-Lahad, S. Zevin, I. Hadas-Halpem, Y. Bar-Ziv, and A. Abrahamov. Preliminary report of low-dose enzyme replacement therapy with human recombinant glucocerebrosidase in Gaucher disease. Am. J. Hum. Genet. 55(supply.All9 (1994). 

B. Friedman, K. Vaddi, C. Preston, E. Mahon, J. R. Cataldo, and J. M. A. McPherson, Comparison of the pharmacological properties of carbohydrate remodeled recombinant and placental-derived /i-Glucocerebrosidase Implications for clinical efficacy in treatment of Gaucher disease, Blood, 93 (1999) 2807-2816. 

Beta-Glucocerebrosidase, recombinant, glycoprotein enzyme purified from human placenta tissues... 

Long-term expression, systemic delivery, and macrophage uptake of recombinant human glucocerebrosidase in mice transplanted with genetically modified primary myoblasts. Hum. Gene Ther. 9, 2375-2384. 

Because of potential limitations in die supply of acceptable placentae and the remote possibility of infective contaminants, it was of great interest to determine whether macrophage-tainted glucocerebrosidase produced from recombinant sources would be as effective as (he natural product We therefore conducted a cnllabnrative atudv with investigators at Mount Sinai School of... 

Medicine in New York using glucocerebrosidase that had been produced lecont-binantly in Chinese hamster ovary cells. The enzyme obtained from these cells was modified by exposure to the- same these cxogtycosidases used previously to produce mannose-terminal placental glucoceiebrosidase (Kg, 2). The clinical effectiveness of the recombinant macrophage-targeted enzyme was found to be... 

Grabowski GA, Barton NW, et al. Enzyme therapy in type 1 Gaucher disease comparative efficacy of mannose-terminated glucocerebrosidase from natural and recombinant sources. Ann Intern Med 122 33-39,1995. 

Recombinant beta-glucocerebrosidase enzyme replacement therapy... 

Alglucerase is a modified form of human /S-glucocerebrosidase. Ceredase is a citrate buffered solution of alglucerase manufactured from human placental tissue. It has largely been replaced by Cerezyme, which is produced by recombinant DNA technology. Both are given intravenously in the treatment of type I Gaucher s disease. 

G. A. Grabowski, N. W. Barton, G. Pastores, J. M. Dambrosia, T. K. Banerjee, M. A. McKee, C. Parker, R. Schiffmann, S. C. Hill, and R. O. Brady. Enzyme therapy in type 1 Gaucher disease comparative efficacy of mannose-terminated glucocerebrosidase from natural and recombinant sources. Ann. Int. Med. 122 33 (1995). 

Recombinant enzymes Gaucher s disease Tobacco Glucocerebrosidase AMT 13,41... 

The adult form of Gaucher disease (type I) is currently the only sphingolipid storage disease for which a causal therapy is available [33]. The patients are treated with a modified glucocerebrosidase from human placenta or a recombinant sample. The protein carbohydrates contain the targeting information for the mannose receptor on macrophages. 

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