Mannose-terminal

M. K. Bijsterbosch, W. Donker, H. Van de Bilt, S. Van Weely, T. J. Van Berkel, and J. M. Aerts, Quantitative analysis of the targeting of mannose-terminal glucocerebrosidase. Predominant uptake by liver endothelial cells, Eur. J. Biochem., 237 (1996) 344-349. 

Grabowski, G.A., N.W. Barton, G. Pastores, J.M. Dambrosia, T.K. Banerjee, M.A. McKee, C. Parker, R. Schiffmann, S.C. HiU, and R.O. Brady, Enzyme therapy in type 1 Gaucher disease comparative efficacy of mannose-terminated glucocerebrosidase... 

R. W. Jansen, G. Molema, T. L. Ching, R. Oosting, G. Harms, F. Moolenaar, M. J. Hardonk, and D. K. F. Meijer, Hepatic endocytosis of various types of mannose-terminated albumins. What is important, sugar recognition, net charge or the combination of these features, J. Biol. Chem. 266 3343-3348 (1991). 

Figure 3 Bar graph showing uptake of glncocengbrosldase by hcpaiocytcs and non-panoichymfll cells isolated from rat liver alter inflnion of 5.7 U of native or 5.0 U of mannose-terminal enzyme. (Adapted from Ref. 22.)...

Rgura 6 Effect of weekly intravenous infusions of mannose-terminal glncocerebrosi-dase on senim acid phosphatase activity. Enzyme was administered as described in Figure... 

Medicine in New York using glucocerebrosidase that had been produced lecont-binantly in Chinese hamster ovary cells. The enzyme obtained from these cells was modified by exposure to the- same these cxogtycosidases used previously to produce mannose-terminal placental glucoceiebrosidase (Kg, 2). The clinical effectiveness of the recombinant macrophage-targeted enzyme was found to be... 

Y. Sato and E Bcutler. Binding, intenvUizatlofi, and degradation of mannose terminated glucocwebtosidase by macrophages, /, Clin. Invest. 91 1909 (1993). 

G. J. Murray, K. L, Oliver, F. 5. Jin, and R. O. Brady. Studies an the turnover of exogenous mannose-terminal glucocciebrosiJase in rat liver lymsomes. S. Cell... 

The clinical effects of mannose-terminal glucocerebrosidase were initially examined in eight patients with non-neuronopathic, type 1 Gaucher disease. Seven of the patients were either adolescents or adults, and one was a 4-year-old boy. A total dose of 190 U of enzyme was administered intravenously to each of these patients weekly over a 6-month period. A beneficial effect was clearly evident only in the youngest of the recipients [26]. A dramatic increase in... 

Figure 2 Production of mannose-terminal glucocerebrosidase by sequential deglycosylation of the native placental enzyme with exoglycosidases. Abbreviations as in Figure 1. (From Ref. 10.)...

Figure 4 Immunogold localization of mannose-terminal glucocerebrosidase in rat liver Kupffer cells 30 minutes after enzyme infusion. Gold particles are concentrated in the lysosomes. Lys = Lysosome M = mitochondrion Nuc = nucleus PM = plasma membrane. Original magnifications (A) x10,000 (B) x33,000. Bars = 1 pm. (From Ref. 24.)...

Figure 5 Effect of weekly intravenous infusions of mannose-terminal glucocere-brosidase on hemoglobin concentration in a 4-year-old boy with type 1 Gaucher disease. Solid black bars along the abscissa represent periods of enzyme treatment. A low dose of enzyme (9-12 U/kg) was administered between weeks 1 and 105 dosage was increased to 30 U/kg between weeks 131 and 320. Shaded areas represent the mean pretreatment and apparent steady-state values observed during the periods of low- and high-dose enzyme supplementation ( 1 SD). Normal range 11-15 g/dL. (From Ref. 10.)...

Figure 9 Effect of mannose-terminal glucocerebrosidase on the bone marrow. Right posterior iliac crest biopsy specimens were obtained before (Pre) and after (Post) 3-1/2 years of treatment with high-dose enzyme supplementation. Marked clearing of the Gaucher cell infiltrate is evident in the posttreatment specimen. Original magnification x8. (From Ref. 28.)...

Figure 11 Enzymatic activity profile of mannose-terminal glucocerebrosidase in plasma of a boy with Gaucher disease. Enzyme was infused over 4 hours at a constant rate of 12.25 U/kg. A steady-state value of 10.6 mU/mL was achieved during the first hour. When the infusion was terminated at 240 minutes, enzyme was cleared from the plasma by a first-oider process with an elimination half-life of 6.3 minutes. The dashed line represents the activity profile calculated from the observed kinetic constants.

G. J. Murray and F. S. Jin. Immunoelectron microscopic localization of mannose-terminal glucocerebrosidase in lysosomes of rat liver Kupffer cells. J. Histochem. Cytochem. 43 149 (1995). 

Mannose-terminating compounds made in connection with glycoprotein work have the sugar either or linked through 0-4 of chitobiose. The first of these reports exemplifies the use of polymer-supported reactions. Chemical and enzymic methods were used in the production of the latter trimer. 

Mannose-terminating compounds to have been described ate 17, which is the Le determinant linked to a linear mannotriose, and 18 with two lactosamine... 

Another interesting application of specific interactions in SAMs is the use of ligand-derivatized thiols to build up complex biosupramolecular architectures via molecular recognition-driven processes. Typical examples include the use of biotin- and mannose-terminated SAMs to biorecognize and assemble streptavidin and concanavalin A protein layers, respectively, on metal surfaces with diverse purposes (Figure 12). " ... 

Dechtrirat, D., et al. Hybrid material for protein sensing based on electiosynthesized mip on a mannose terminated self-assembled monolayer. Adv. Funct. Mater. 24(15), 2233-2239 (2014)... 

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