Reagent kits

Apart from innovative work, RMs are essential during exerdses such as the introduction to a laboratory of a method from elsewhere or the transfer of an established method onto new instrumentation. Even where the conditions for the analysis have been standardized by the manufacturer of a reagent kit, some validation work should still be undertaken so as to have documented data for quality assurance purposes, e.g. accreditation, as a basis for IQC, for later reference when problems which may be related to equipment, reagents or staff etc. need to be investigated. 

For an active ingredient in a radioactive drug product, except for nonradioactive reagent kits, the reserve sample shall be retained for ... 

The Bioscience Division supplies reagents, kits and other enabling technologies and products for R D in life science. The majority of the product line is based on microp-orous membrane, ultrafiltration, and chromatographic technologies. 

For the frozen cell/tissue samples, RNA extraction was carried out by using the TRIzol reagent kit. For the paraffin-embedded cell/tissue, RNA extraction was carried out by two methods heating and nonheating using enzyme digestion for comparison. RT-PCR was performed to compare the results. A... 

BCA Protein Assay Reagent Kit (Pierce) or Bicinchoninic Acid Kit (Sigma) containing ... 

Adolor Corporation currently possesses a 300-member custom TFP reagent kit that continues to increase in number. Focused libraries are produced in as little as 2-3 days by derivatizing an amine with a subset of the reagents. Analytical data are collected on all the members of the library. Focused libraries find utility in SAR exploration and patent protection.2 For larger library campaigns, which require 3-4 weeks to complete,... 

Where possible, commercial sources of reagents, kits, and equipment are listed throughout the text instead of in a separate index. Extensive references are provided to facilitate the task for those readers who may wish to consult the literature for additional information on specific topics. All books can be improved, and this volume is no exception. I welcome constructive criticism from my colleagues and students. With this help I look forward to offering a greatly improved second edition. 

Chemiluminescence of oxidized luminol has been the basis of several lumino-metric methods of estimation of TAC (Table 1). The mostcommon is to measure the induction time of the reaction. Often the chemiluminescence is first induced by an oxidant and then attenuated by addition of a sample, and the time to recover the initial fluorescence is measured. The enhanced chemiluminescent assay introduced a decade ago is based on the oxidation of luminol by perborate or by hydrogen peroxide in a reaction catalyzed by horseradish peroxidase. Enhancement (and stabilization) of luminescence is achieved by addition of p-iodophenol. The original procedure used a commercial reagent kit (ECL Anti-oxidant Detection Pack... 

Currently available reagent kits, which help to remove impurities (such as nucleic acids, lipids, and carbohydrates) but which do not modify properties of proteins while detecting even trace amounts of proteins in samples, enable analysts to obtain pure proteins. Such kits are often used to prepare proteins for their separation using 2D electrophoresis, which is typically a preliminary step in the proteomics research methods. 

We have measured FSH in unextracted urine on an AxSYM random-access immunoassay analyzer (Abbott laboratories, Abbott Park, IL) with a MEIA (microparticle enzyme immuno assay) reagent kit. In order to correct for dilution, creatinine was measured, and the urinary FSH was normalized for creatinine concentration. Urine and serum samples were obtained from 40 women between 32 and 55 years of age. All women were healthy, except for a benign gynecological illness for which they were admitted to our hospital. All women had normal renal function. On the day of operation, we took six serum samples from each patient, each at least an hour apart, in order to calculate the mean serum FSH concentration. During the same day, we collected an early-morning urine sample, 24-h urine sample, and a random void urine sample. 

Henderson, J., M. O Malley, J. Sanborn, M. Verder-Carlos and B. Wilson (1998). Comparison of Acetylcholinesterase Assays Run Under Conditions Specified by the Standard Ellman Method and Conditions Specified by a Commercial Cholinesterase Reagent Kit, Department of Pesticide Regulation, Worker Health and Safety Branch Report HS-1752, Sacramento, CA, USA. 

Under most assay conditions, ADH is reasonably specific for ethanol. Interference, relative to ethanol, is generahy about 7% for isopropanol, 3% for methanol, and 4% for ethylene glycol. Reagent kits for use with manual spectrophotometers or automated analyzers are available from several manufacturers. Some manufacturers (Dade-Behring [flcfl], Abbott [TDx], Dade-Behring [EMIT]) claim interference from isopropanol, methanol, acetone, and ethylene glycol to be less than 1%. 

Beta-endorphin is a frequently measured opioid peptide. Immunoassay is the method of choice for the analysis of plasma (3-endorphin. Both RIAs and direct IRMAs have been developed for this purpose. Commercial reagent kits are widely available, and many commercial reference laboratories offer P-endorphin assays. The concentrations of P-endorphin are usually very low to undetectable in normal subjects, and it may be necessary to use extraction procedures to detect meaningful concentrations in plasma. The specificity of commercial antibodies for p-endorphin relative to p-LPH varies widely in some immunoassays, 50% cross-reactivity is seen with p-LPH. With polyclonal antibodies, results may be spuriously high owing to crossreactivity with serum immunoglobulin G (e.g., in patients with immunoglobulin G myeloma). 

Near the end of incubation, prepare serial dilutions of phosphate standard from the ColorLock reagent kit, in water. Dispense 6 pi of each concentration (100, 50, 25, 12.5, 6.25, 3.13, 1.56, 0 pM) in quadruplicate into the 1,536-well assay plate holding the assay mixture under incubation. For 0 pM prepare 16-32 replicate wells for sufficient sample size in subsequent data analysis. 

Several crystallization-screening protocols have been developed over the past decade to help identify initial crystallization conditions. These include footprint screening (Sutra et al., 1992), sparse matrix sampling (Jancarik and Kim, 1991), statistical methods (Carter and Carter, 1979), and MON-48 (Shieh et al., 1991). Reagent kits for some of these screens are now commercially available (Hampton Research [www.hamptonresearch.com], Emerald BioStructures [www.emeraldbio-structures.com]) and are commonly used in the laboratory. 

Immobilized reagents for bioluminescent analysis have their peculiarities. For example, immobilization reagent kit should contain all the reaction components, and the procedure must provide opportunities for coimmobilizing luciferase with other enzymes and their substrates. In addition, methods for production of immobilized bioluminescent reagent must also meet the following requirements. 

Thermal cycling polymerase chain reaction (PCR) machine and reagents for performing PCR GeneAmp PCR Reagent Kit (Perkin-Elmer, Foster City, CA). 

---