Hampton Research

Paraffin oil (Hampton Research or Molecular Dimensions) Silicone oil (Hampton Research or Molecular Dimensions) Microbatch plates (e.g. Nunc, Terazaki, Douglas Instruments)... 

Precipitate solution at 5% higher concentration than that in the drops Micro tools (Hampton Research)... 

Items from Protocol 4.1, with the exception of propane Crystal wand (available from Hampton Research, Inc.) Cryotongs (available from Hampton Research, Inc.)... 

Vial clamp (available from Hampton Research, Inc.)... 

Hampton Research Ltd) 9. Examine the drops for the presence of crystals... 

Hampton Research Ltd) 6. Stir the liquid around the crystal upwards with a loop. 

Pins and loops (Hampton Research Ltd or Molecular 7. Pick a crystal with the loop, pass through a solution of... 

The general approach to crystallizing a protein is to first use commercially available screens containing a broad spectrum of conditions, such as those provided from Hampton Research [5, 6] (http //www.hamptonresearch.com) and Emerald BioStructures (http //www.decode.com/emeraldbiostructures), and then fine-tune the conditions by systematically adjusting the concentrations of protein and precipi-tants, varying pH values, additives, temperature and other conditions. However, one of the parameters that is often ignored is the protein sequence itself. 

Hampton Research, available online at http // www.hamptonresearch. com,accessed on October 9,2001. 

FIGURE 2.2 A collage of protein crystals, ranging from the large and perfect to the small and flawed. Courtesy of Hampton Research. 

FIGURE 2.10 An array of commercially available and commonly used plastic plates for both sitting and hanging drop vapor diffusion crystallization. Also in the picture is a box of silicone coated cover slips for hanging drops. Courtesy of Hampton Research. 

Several crystallization-screening protocols have been developed over the past decade to help identify initial crystallization conditions. These include footprint screening (Sutra et al., 1992), sparse matrix sampling (Jancarik and Kim, 1991), statistical methods (Carter and Carter, 1979), and MON-48 (Shieh et al., 1991). Reagent kits for some of these screens are now commercially available (Hampton Research [www.hamptonresearch.com], Emerald BioStructures [www.emeraldbio-structures.com]) and are commonly used in the laboratory. 

Capillaries, cryo-loops, and other crystal mounting supphes are available from Charles Supper Company [www.charles-supper.com] and Hampton Research... 

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