Polymerase chain reaction thermal cycling

Yoon DS, Lee Y-S, Lee Y, Cho HJ, Sung SW, Oh KW, Cha J, Lim G (2002) Precise temperature control and rapid thermal cycling in a micromachined DNA polymerase chain reaction chip. J Micromech Microeng 12 813-823... 

Polymerase chain reaction (PCR) is one of the most important techniques for rapid bacterial identification. It consists of repeated cycles of enzymatic reactions in a thermal cycler (PCR machine) that copies DNA strands many times. The DNA amplified in one PCR cycle is used as a template for the next cycle. This results in an exponential increase of the desired target... 

A thermal cycler (see 8.2.3.2 Polymerase Chain Reaction) is required for the cycle sequencing reaction. For fluorescent cycle sequencing we recommend instruments from Applied Biosystems (e.g. the 16-capillary ABI PRISM 3100 Genetic Analyser) other instruments are available from GC Healthcare and Beckman. For detailed instructions, refer to the respective user s manual or chemistry guide. 

Micro-reaction chambers for the thermal cycling of DNA amphfication by the polymerase chain reaction (PCR) in silicon by bulk machining using anisotropic wet etching have been fabricated by Moore and co-workers [40]. The rapid temperature cycling and the small size of the chambers may be combined with an appropriate transducer in a miniaturized analytical system. 

Thermal cycling polymerase chain reaction (PCR) machine and reagents for performing PCR GeneAmp PCR Reagent Kit (Perkin-Elmer, Foster City, CA). 

Park, N., S. Kim, and J.H. Hahn. Cylindrical compact thermal-cycling device for continuous-flow polymerase chain reaction. Anal Chem. 2003, 75(21), 6029-6033... 

DNA polymerase has been entrapped within POPC extruded vesicles, together with a template DNA, primers, and dNTPs. Thermal cycles allow the polymerase chain reaction occurs within vesicles. 

Chapter 36 describes a protocol for an isothermal chain reaction that makes use of the presence of DNAprimere, T7 promoters, T7 polymerase, RNaseH, and AMV reverse transcriptase to produce large quantities of nucleic acid when the primers used match an RNA (or DNA) template. This system has the advantage that no thermal cycling block is required, and hence the number of samples that can be processed daily is not limited by the availability of specialized equipment. 

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