Rats, liver tissue

Binding affinity inhibition constant X (tiM) versus [ HJoxytocin in rat uterine tissue. Binding affinity inhibition constant Kj (tiM) versus [ HJarginine vasopressin in rat liver tissue. Binding affinity inhibition constant AT (tiM) versus [ H] arginine vasopressin in rat kidney tissue. 

Binding affinity IC50 (nM), concentration required for half-maximal inhibition of binding of [ H]arginine vasopressin to rat liver tissue. 

In rat liver tissue, p-cresol was 5- to 10-fold more toxic than the o- or -isomers as determined by the degree of cell killing. Furthermore, the toxicity ofp-cresol was dependent on the formation of a reactive intermediate, and it was suggested that the mechanism of toxicity for p-cresol may differ from that of the o- and 7 -isomers. 

Chang H et al Effects of phenoxyacetic acids on rat liver tissues. J Agric Food Ghent 22 62-65, 1974... 

Reductive cleavage of 4-dimethyl-aminoazobenzene by rat liver tissue. Intracellular distribution of the enzyme system and its requirement for triphosphopyridine nucleotide. Journal of Biological Chemistry, 180, 1125-1136. 

Rat liver tissue (CDDs) Grinding of sample with Na2S04 addition of [13C]2,3,7,8-TCDD and OCDD Soxhlet extraction with toluene volume reduction addition of hexane clean-up with column chromatography HRGC/LRMS (EI/SIM) 100-250 pg No data Van den Berg et al. 1989... 

Golor G, Neubert D. 1989. EROD (ethoxyresorufin-o-deethylase) activity in rat liver tissue after application of TCDD and OCDD alone and in combination [Abstract]. Naunyn Schmiedeberg s Arch Pharmacol 339 10. 

We had previously eliminated the possibility that retinol affected bacterial viability in this assay. The likelihood of a non-specific inhibitory effect of retinol was also eliminated, since there was no effect of vitamin A alcohol on mutagenicity induced by adriamycin, a direct-acting mutagen in Salmonella (23). Thus, these findings demonstrated that retinol inhibited the metabolism of 2-fluorenamine by rat liver tissue preparations to forms capable of producing mutations in S typhimurium. 

DeDuve C, Pressman BC, Gianetto R, Wattiaux R, Appehnans F. Tissue fractionation studies 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem. J. 1955 60 604. 

B14. Berthet, J., and de Duve, C., Tissue fractionation studies. 1. The existence of a mitochondria-linked, enzymically inactive form of acid phosphatase in rat-liver tissue. Biochem. J. 50, 174-181 (1951). 

As in other organs, ABCBl is the best investigated transporter in the liver. It was demonstrated that turpentine-induced APR leads to a 50-70% reduction of ABCBl expression and function in rat liver tissue 48 h after treatment [103]. Similar results were observed in studies of endotoxin-triggered inflammation in which both constitutive and induced expression were affected in rodents [104]. Further experiments have shown that reduction of ABCBl expression is also linked to a reduction in ABCBl function. In two distinct experimental setups, Shiga-toxin II and endotoxin administration to rats prompted a substantial reduction in ABCBl function (assessed by hepatobiliary doxorubicin and "TC-sestamibi clearance), accompanied by a significant reduction in ABCBl protein expression [69, 105]. Likewise, endotoxin-treated mice displayed decreased liver-mediated doxorubcin clearance as a result of... 

Hilali A, Crutzen-Fayt MC, Gerber GB. 1993. Effect of age on the ability of rat liver tissue to transform chemical promutagens to mutagens. Gerontology 39 125-127. 

Riis B, Risom L, Loft S, Poulsen HE (2002) Increased rOGGl expression in regenerating rat liver tissue without a corresponding increase in incision activity. DNA Repair 1 419-24... 

Figure 2. ElVect of treatment with thia fatty acids on mitochondrial 3-oxidation. The values represent the mean SD for four animals in each experimental group. Measurements performed in E-fractions of rat liver tissue. All values given as percentage of control (CMC). The rats were treated with a dose of 150 or. 300mg/d/Kg body mass for 7 days.

In this procedure cell monolayers are detached from culture vessels by trypsin-ization or scratching using a rubber policeman and pelleted by centrifugation. Isolated nuclei and nuclear envelopes are pelleted by centrifugation. From whole organs such as rat liver, tissue cubes of about 1 mm are dissected. The pellets or tissue cubes are then treated in the following manner ... 

The most coinprehcasive study on the variation with age in the hepatic levels of vitamin A in rats has been made by Guerrant (1949). The investigation included a total of 59 animals ranging in age from 1 to 749 days. The analyses of liver samples from newhom rats showed a rather low vitamin A content of the tissue, the average value observed for 8 animals being 20 I.U./gm. This concentration is in accord with the values reported by Williamson (1948) for fetal rat liver tissue and by Dann (1932) and Henry et al. (1949) for livers of newborn animals. 

Fig. 2. Variation with age in vitamin A concentration of rat liver tissue. The curve is constructed on the basis of data reported by Guerrant (1949).

Okino et also demonstrated dmg permeability into tissues from in situ forming hydrogels. Photopolymerized hydrogels based on styrene-derivatized gelatin were formed on the surface of rat liver tissue and rhodamine-albumin was used as a dmg model to characterize dmg release and diffusion into the tissue. This work showed the multitude of variables inherent to photopolymerizable systems to control dmg release characteristics. 

The antitubercular activity of isoniazid (5, Fig. 17) prompted studies on its metabolic fate. In man, the major metabolite was found to be the acetylated derivative, acetylisoniazid (6, Fig. 17). Acetylisoniazid is known to undergo further transformation in vivo as outlined in Fig. 17 (555). In rats it was shown that acetylhydrazine radiolabeled in the acetyl group can covalently bind to rat liver tissue protein 354). In the case of salicyloylhydrazide and 1-... 

Fig. 5. Apparent half-life of mt DNA in rat liver tissues of different growth rates. M SD of 10 values each, t/i values were measured in rat liver tissues of different growth rates. The growth rate is estimated in days during which the specific activity of isolated n DNA is diluted to 50% by newly formed, unlabeled n DNA. mt DNA in livers from mature rats show a t/J of 7-9 days. But when very young animals are used much longer half-lives are found. In rapidly growing liver tissue mt DNA apparently has little or no turnover. ...

In normal and regenerating rat liver tissue, /8-D-2-acetamido-2-deoxygluco-sidase has been shown to be localized primarily in the fraction of light mitochondria, enriched with lysosomes. The interaction of rat liver )8-d-2-acetamido-2-deoxyhexosidase with agarose-immobilized ovalbumin has been investigated. The use of the latter as an aflfinity chromatography material was also studied. 

Working with hypertonic sucrose homogenates, Kennedy and Lehninger found that isolated washed mitochondria of rat liver tissue oxidized not only succinate but also other key intermediates of the Krebs cycle. They also reported that fatty acid oxidation was localized in the mitochondria and furthermore demonstrated that phosphorylations coupled to these oxidations took place in these preparations, as could be shown with tracer phosphate experiments. 

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