Chromatography materials

Gel-filtration chromatography materials such as Ultrogel AcA44 separate molecules on the basis of molecular size. Larger protein molecules are unable to penetrate the small pores in this material and pass through the column more rapidly than the smaller protein molecules. 

Element Chromatography Material Detection method Detection limit Ref. 

Chromatography. Materials extracted from the recycle cup deposit using a proprietary technique provide insight as to the organic molecules that contribute to fouling. GC/MS and LC/UV spectroscopy were used to analyze the extracts. 

The introduction of gel permeation chromatography materials and ultrafiltration membranes has provided powerful techniques which give very good fractionations on the basis of molecular size. Both techniques are now widely used, and although less popular, centrifugation is available as an alternative independent technique. 

HiUman, D.E., Morris, PR., Paul, J.I. and Pickles, D. (1977) Comparison of the modes of degradation of viscosity index improvers in the Kurt Orbahn and EZG tests by gel permeation chromatography. Materials Quality Assurance Directorate Technical Paper No. 677, London. 

One type of affinity-chromatography material of particular interest in carbohydrate chemistry is the insolubilized derivative of concanavalin A, a phytohemagglutin that shows specificity towards certain carbohydrates. 

Table 3 Use of agarose cyclic imidocarbonates for the preparation of affinity chromatography materials without linkage extension...

Intact phycobilisomes were dissociated in 5 mM potassium phosphate, 1 mM sodium azide, 2mM EDTA, ImM Phenylmethane-sulfonyl fluoride (PMSF), pH=7.05 To separate the resulting complex by two different anion-exchange chromatography materials, Cellex-D and Q-Sepharose, a phosphate gradient was used at pH = 7.05 and pH = 8 respectively. (Fuglistaller,P., Suter, F., Zuber H. (1986) Biol. Chem. Hoppe-Seyler 367, 601-6l 4) Subsequent to chromatographic separation the APC I fractions were pooled. 

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