Rabbit collagen

Another 3-D cornea model, comprising rabbit primary cultures of epithelial and stromal cells as well as mouse immortalized endothelial cells, was described in 1994 by Zieske and coworkers [70], They showed the influence of endothelial cells on the formation of a tightly packed, multilayered epithelium as well as the expression of laminin, type VII collagen, a6 integrin, keratin K3, and a-enolase. Furthermore, their findings suggested that the formation of an in vivo-like epithelium requires the cultivation of the 3-D corneal construct under AIC conditions. By contrast, LCC methods of cultivating corneal equivalents in the absence of endothelial cells failed to promote the expression of differentiation markers and basement membrane components. 

Feverfew extract was shown to inhibit deposition, aggregate formation and spreading of platelets on collagen fibres [54]. Changes in membrane-cytoskeletal interaction leading to a change in expression of membrane receptors involved in these processes may explain this result. Feverfew extract was further able to protect the endothelial monolayer in rabbit aortas from perfusion injury and some reversible increase in cAMP levels in the aorta segments was found [55]. 

U size into rats produced reticulin nodules that later developed into areas of dense collagenous fibrosis." The latter alumina by the same route in mice and guinea pigs caused development of a reticulin network with occasional collagen, whereas in rabbits only a slight reticulin network was observed. Intratracheal administration of another form of alumina in rats, corundum of particle size less than Ip, caused the development of compact nodules of reticulin. 

Platelets aggregation stimulation. Fruit juice, administered intravenously by infusion to dogs at a dose of 5 mL/min, was active. Total infusion was 300 mL h Oil, administered orally to six New Zealand white rabbits fed a commercial diet supplemented with 60 g/kg of coconut oil low in all PUFA for 60 days, produced a platelets aggregation induced by both thrombin and collagen significantly lower with either fish or linseed oil (n-3 PUFA), than with corn oil (n-6 PUFA) or the low PUFA coconut oil . 

Collagen sponges with microporous structures from tilapia were fabricated reconstituted collagen fibrils using freeze-drying and cross-linked by DHT treatment or additional treatment with WSC treatment. The pellet implantation tests into the paravertebral muscle of rabbits demonstrated that tilapia collagen caused rare inflammatory responses at 1- and... 

Yata, N., et al. 1985. Enhanced rectal absorption of sodium ampicillin by TV-acyl derivatives of collagen peptide in rabbits and rats. J Pharm Sci 74 1058. 

Pleyer, U., et al. 1992. Use of collagen shields containing amphotericin B in the treatment of experimental Candida albicans-induced keratomycosis in rabbits. Am J Ophthalmol 113 303. 

Fig. 18. Bacterial infection observed during percutaneous implantation of silicone devices into rabbits (O) non-treated and ( ) collagen-immobilized...

In rabbit platelets, inhibited Ca influx and collagen-induced aggregation inhibited induced arachido-nate release 617... 

Recently, there has been in the use of other pharmacologic agents to modify the chronic total occlusion and render it more suitable for treatment (38-40). An animal model has been developed for testing these new approaches. In this rabbit model, thrombin is injected into an isolated portion of the femoral artery. After recovery, during the next two to four month, the thrombus is replaced by collagen which results in a chronic total occlusion. 

Figure 3.29. Relationship between proteoglycans and collagen fibrils in tendon. Transmission electron micrograph showing positive staining pattern of type I collagen fibrils from rabbit Achilles tendon stained with quinolinic blue. Proteoglycan filaments (arrows) are shown attached to collagen fibrils.

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