Quinoprotein glucose dehydrogenase

E. D Costa, J. Higgins, and A.P. Turner, Quinoprotein glucose dehydrogenase and its application in an amperometric glucose sensor. Biosensors 2, 71—87 (1986). 

M. Yamada, M. Elias, K. Matsushita, C.T. Migita, and O. Adachi, Escherichia coli PQQ-containing quinoprotein glucose dehydrogenase its structure comparison with other quinoproteins. Biochim. Biophys. Acta Proteins Proteomics 1647, 185-192 (2003). 

Cozier, G. E., and Anthony, C. (1995a). Structure of the quinoprotein glucose dehydrogenase of Escherichia coli modelled on that of methanol dehydrogenase from Methylobact-erium extorquens. Biochem. J., 312, 679-685. 

Yamada, M., Inbe, H., Tanaka, M., Sumi, K., Matsushita, K., and Adachi, O., 1998, Mutant isolation of Escherichia coli quinoprotein glucose dehydrogenase and analysis of crucial residues Asp-730 and His-775 for its function. /. Biol. Chem. 273 22021922027. 

Quinoprotein glucose dehydrogenase and recombinant tyrosinase from Streptomyces antibioticus were immobilized on polyvinyl alcohol and coupled to a Clark oxygen electrode. LOD was 5 nM for dopamine (10a), L-dopa (10b) and adrenaline (epinefrine, 15a). An electroimmunological biosensor for p-cresol was developed, based on the... 

Fig. 38. Variants of glucose conversion and indication of the resulting reaction effects using biosensors. GOD glucose oxidase, GDH glucose dehydrogenase, GDH(PQQ) quinoprotein glucose dehydrogenase, HK hexokinase, Afox oxidized mediator, JWW reduced mediator.

Mullen et al. (1985) circumvented the problem of oxygen demand by using quinoprotein glucose dehydrogenase from Pseudomonas or Acme-... 

J. A. Dijkstea, D. W. 1999 EMBOf 18, 5187-5194 Structure and mechanism of soluble quinoprotein glucose dehydrogenase. 

Heller A 1993 High current density wired quinoprotein glucose dehydrogenase electrode Anal. Chem. 65 238-41... 

Laccase (Lacc) (300 - 500 U mg ) from Coriolus hirsutus was purified and supplied by the Research Center for Molecular Diagnostics, Moscow (Russia). Quinoprotein glucose dehydrogenase (GDH) (950 U mg - ) from Acinetobacter calcoaceticus, p-aminophenol (PAP) and p-aminophenol phosphate (PAPP) was kindly supplied by Boehringer Mannheim. All chemicals were used without further purification. 

An ultrasensitive biosensor for these phenolic substances and redox dyes has been created with the quinoprotein glucose dehydrogenase and laccase, both of high specific activity coentrapped in a gel matrix in front of a Clark-type oxygen electrode. 

Zhao, C.A. and Wittstock, G. (2004) Scanning electrochemical microscopy of quinoprotein glucose dehydrogenase. Analytical Chemistry,16, 3145-3154. 

Iswantini D, Kano K, Ikeda T (2000) Kinetics and thermodynamics of activation of quinoprotein glucose dehydrogenase apoenzyme in vivo and catalytic activity of the activated enzyme in Escherichia coli cells. Biochem J 350 917-923... 

Enhancement of the Stability of Wired Quinoprotein Glucose Dehydrogenase Electrode... 

In our previous paper (7), we reported the high current density wired quinoprotein glucose dehydrogenase GDH electrode. In addtion to the extraordinarily high current density, the total insensitivity to oxygen and the wide pH operation range make the wired GDH electrode very attractive. However the reported half life time of the GDH electrode was only 8 hours. In the present paper, we report the results of the study for enhacing the operational stability of the wired GDH electrode. 

Elias MD, Nakamura S, Migita CT, Miyoshi H, Toyama H, Matsushita K, Adachi O, Yamada M (2004) Occurrence of a bound ubiquinone and its function in Escherichia coli membrane-bound quinoprotein glucose dehydrogenase. J Biol Chem 279(4) 3078—3083... 

Dietzen DJ, Wilhite TR, Rasmussen M, Sheffield M (2013) Point-of-care glucose analysis in neonates using modified quinoprotein glucose dehydrogenase. Diabetes Technol Ther 15 (ll) 923-928... 

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