Glucose analysis

Glucose. There are four basic principles utilized for glucose analysis. The most widely used depends upon the reduction of some element such as copper or iron. This is the principle of the Folin-Wu method, the ferricyanide method, used on the Autoanalyzer, and the cupreine ( ) method used on the SMA-12/60. 

A second principle used widely for glucose analysis, is that of the oxidation of glucose enzymatically, mediated by the action of glucose oxidase with the formation of gluconic acid and hydrogen peroxide (22). In this procedure it is the hydrogen peroxide which is usually assayed for determination of glucose. This method suffers from the action of inhibitors which occur, particularly with patients in a diabetic coma and these need to be removed. 

Comparison of the various hexokinase enzyme mixtures for specificity in glucose analysis... 

This problem removes the cupreine method as an acceptable procedure for the Pediatric Laboratory for glucose analysis under any circumstances ... 

Glucose oxidase Aspergillus rilger Blood glucose analysis 4.6... 

These biosensors were tested for glucose and lactate measurements in sera, and for lactate measurements in whey solutions. Good agreements were obtained between the present method and reference methods. For glucose analysis in serum, the coefficient of variation for 53 repeated measurements performed over a 10 h period was 4.8% while for lactate analysis, 80 assays performed over a 15 h period gave a coefficient of variation of 6.7%. Thus,... 

These differences emphasize potential metabolite interference related to methodology. An interference can occur when one method of analysis is used, but not with another. These possible differences are well appreciated for glucose analysis. Caraway (C4) has shown that the apparent... 

Analyses of glucose and fructose in musts of commercially processed fruit have been made for several years (18). Glucose oxidase (Worthington Glucostat reagent) has been the method of choice for glucose analysis. Acid diphenylamine reagent (24), which is specific for the keto... 

This consists of fruit immersed in a sugar syrup prepared with saccharose, with or without glucose. Analysis of such products usually comprises the determinations made with crystallised fruit (q.v.), and a few hints may be given with reference to the determination of the sugars. 

Chaiken J, Finney W, Knudson PE, Weinstock RS, Khan M, Bussjager RJ, Hagrman D, Hagrman P, Zhao YW, Peterson CM, Peterson K. Effect of hemoglobin concentration variation on the accuracy and precision of glucose analysis using tissue modulated, noninvasive, in vivo Raman spectroscopy of human blood a small clinical study. Journal of... 

DNLM 1. Blood Glucose-analysis. 2. Blood Glucose Self-Monitoring. 

Baca JT, Feingold DN, Asher S A. Tear glucose analysis for the noninvasive detection and monitoring of diabetes mellitus. Ocular Surface 2007, 5, 280-293. 

Figure 7.21 Determination of the structure of Bacillus amyloliquefaciens a-amylase limit dextrin, using enzymes no reaction with (3-amylase (b) reaction with pullulanase to give maltose + maltotriose (c) reaction of glucoamylase to give two tetrasaccharides, both ofwhich are eventually converted into panose + glucose. Analysis of the reactions can be made by thin layer chromatography239.

Metabolic blood parameters are assayed in anesthetized male rats using a modified method of glucose clamp studies in rodents (Terrettaz and Jeanrenaud 1983). Four to 6 rats per group (vehicle control and one dose of the candidate compound) are used. Rats are anesthetized with an intraperitoneal injection of pentobarbital sodium (60 mg/kg), tracheotomized, and one jugular vein per rat is cannulated for intravenous infusion the other vein is prepared for collection of blood samples. Anesthesia is maintained for up to 7 hours by subcutaneous infusion of pentobarbital sodium (adjusted to the anesthetic depth of the individual animal about 24 mg/kg/h). Body temperature is monitored with a rectal probe thermometer, and temperature is maintained at 37 °C by means of a heated surgical table. Blood samples for glucose analysis (10 il) are obtained from the tip of the tail every 15 minutes, and for lactate analysis (20 p.1) every... 

The oldest example of an integrated dry reagent chemistry for quantitative analysis of a metabolite is the Dextrostix reagent strip (Miles Dia ostics) for whole blood glucose analysis. The cross section of the element is illustrated in Fig. 8a. The detection chemistry is the well-known glucose oxidase-peroxidase procedure. Approximately 50 pL of whole blood is applied to the surface of the... 

Fig. 8. Cross sections and chemistries of test elements for blood glucose analysis, (a) Miles Diagnostics, Inc., (Dextrostix reagent strips), b) BMC, and (c) Fuji.

Sazama K, Robertson EA, Chesler RA. Is antiglycolysis required for routine glucose analysis Chn Chem 1979 25 2038-9. 

For determination of the metabolites in human blood, samples were collected from veins into heparinized or EDTA containing tubes. For glucose analysis, NaF... 

Xie et al. (1994) manufactured a microbiosensor with an integrated thermopile on a quartz chip because of the lower heat conductivity of quartz compared to silicon. The size of the whole sensor was 25.2 x 14.8 x 0.6 mm, and immobilized enzymes (CPG beads) were were placed in the chips microchannels. The sensor was applied to glucose analysis in the 2 to 25 mmol/1 range, using only 1 pi samples. Due to clinical analysis requirements, the sensor might be interesting for blood glucose measurements. 

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