Escherichia coli model

Cozier, G. E., and Anthony, C. (1995a). Structure of the quinoprotein glucose dehydrogenase of Escherichia coli modelled on that of methanol dehydrogenase from Methylobact-erium extorquens. Biochem. J., 312, 679-685. 

Studies of digoxin in an Escherichia coli model of genotoxicity were inconclusive. 

Hydrogen peroxide is mutagenic in Salmonella and Escherichia coli models. 

This is not the place to expose in detail the problems and the solutions already obtained in studying biochemical reaction networks. However, because of the importance of this problem and the great recent interest in understanding metabolic networks, we hope to throw a little light on this area. Figure 10.3-23 shows a model for the metabolic pathways involved in the central carbon metabolism of Escherichia coli through glycolysis and the pentose phosphate pathway [22]. 

More than 30 years ago Jacob and Monod introduced the Escherichia coli lac operon as a model for gene regulation. The lac repressor molecule functions as a switch, regulated by inducer molecules, which controls the synthesis of enzymes necessary for E. coli to use lactose as an energy source. In the absence of lactose the repressor binds tightly to the operator DNA preventing the synthesis of these enzymes. Conversely when lactose is present, the repressor dissociates from the operator, allowing transcription of the operon. 

FIGURE 17. 33 A model of the flagellar motor assembly of Escherichia coli. The M ring carries an array of about 100 motB proteins at its periphery. These juxtapose with motA proteins in the protein complex that snrronnds the ring assembly. Motion of protons throngh the motA/motB complexes drives the rotation of the rings and the associated rod and helical filament. 

If we can develop accurate quantitative models that simulate how cells respond to various enviromnental changes, we can better utilize the chemical synthesis capabilities of cells. Steps toward this goal are being taken. Models of the common gut bacterium Escherichia coli have been developed from mechanisms of subcellular processes discovered or postulated by molecular biologists. These models have progressed to the point where they can be used with experiments to discriminate among postulated mechanisms for control of subcellular processes. 

Perfetto EM, Gondek EK. Escherichia coli resistance in uncomplicated urinary tract infection a model for determining when to change first-line empirical antibiotic choice. Manag Care Interface 2002 6 35M2. 

J. V. Rodriguez, J. A. Kaandorp, M. Dobrzynski, and J. K. Blom, Spatial stochastic modelling of the phosphoenolpyruvate-dependent phosphotransferase (PTS) pathway in Escherichia coli, Bioinformatics 22, 1895 (2006). 

Characterization of Anti-Infective Biological Activity. Acute sepsis models utilizing either Escherichia coli or Staphylococcus aureus intraperitoneal challenge were developed to evaluate the anti-infective properties of PGG in mice. 

Skandamis, P.N. and Nychas, G.J. (2000) Development and evaluation of a model predicting the survival of Escherichia coli 0157 H7 NCTC 12900 in homemade eggplant salad at various temperatures, pHs, and oregano essential oil concentrations . Applied and Environmental Microbiology, 66, 1646-1653. 

The first LAPS utilized silicone nitride (S3N4) as a pH-sensitive layer [68], A light-addressable high resolution pH imaging sensor was applied to the detection of spatially resolved metabolic activity of Escherichia coli colonies on agar medium [69], For a silicone substrate thickness of 20 pm the reported spatial resolution was about 10 pm. The observed pH distribution was in good agreement with the results of simulation based on a two-dimensional diffusion model. 

The Koenigs—Knorr reaction109,130 of the halide 112 with methyl 2,3-di-O-acetyl-jS-D-ribofuranoside (123) in 3 1 (v/v) benzene—1,4-dioxane in the presence of silver carbonate gave a low yield of the disaccharide 124 (see Scheme 34). Compound 124 and its deacetylation and deesterification products, 125 and 126, were used as model compounds for 1H- and 13C-n.m.r. studies47,107 of the KDO-containing exopolysaccharides from Escherichia coli strains LP 1092 and 06 K13 H1 (refs. 84 and 86). 

E. M. T. El Mansi, G. C. Dawson, and C. F. A. Bryce, Steady state modeling of metabolic flux between the tricarboxylic cycle and the glyoxylate bypass in Escherichia coli. CABIOS (now Bioinformatics) 10(3), 295 299 (1994). 

C. Chassagnole, N. Noisommit Rizzi, J. W. Schmid, K. Mauch, and M. Reuss, Dynamic modeling of the central carbon metabolism of Escherichia coli. Biotechnol. Bioeng. 79(1), 53 73 (2002). 

Bartolucci, S., A. Guagliardi, E. Pedone, D. De Pascale, R. Cannio, L. Camardella, M. Rossi, G. Nicastro, C. de Chiara, P. Facci, G. Mascetti, and C. Nicolini. 1997. Thioredoxin from Bacillus acidocaldarius characterization, high-level expression in Escherichia coli and molecular modelling. Biochem J 328 277-285. 

Piperacillin- tazobactam In vitro anti-infective effect of piperacillin-tazobactam (PIP-TZB) combinations on Escherichia coli showed through a PK/PD model that for these combinations, three-times-a-day administration is as effective as four times a day. Pharmacodynamic activity of the combinations can be prolonged by sufficiently high inhibitor concentrations... 

Laux, D. C., McSweegan, E. F., and Cohen, P. S. (1984). Adhesion of enterotoxigenic Escherichia coli to immobilized intestinal mucosal preparations A model of adhesion to mucosal surface components. /. Microbiol. Methods 2, 27-39. 

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