Coriolus hirsutus

Tatiana V, Pegasova P, Zwart O, Koroleva V, Stepanova EV, Rebrikovc DV and Lamzinb V. 2003. Crystallization and preliminary X-ray analysis of a four-copper laccase from Coriolus hirsutus. Acta Crystallogr... 

Most of the degradation products shown in Figure 1A were also detected in the degradation of the 3-0-4 dimers by other white-rot fungi, Coriolus versicolor (29,30) and Coriolus hirsutus 31). 

Figure 1. /3-0-4 lignin substructure model dimers 1-4 and their degradation by white-rot fungi, Phanerochaete chrysosporium, Coriolus versicolor, and Coriolus hirsutus. The ether bond between the C/ and the B-aromatic nucleus is referred to as /3-0-4 bond in lignin chemistry. 

The redox potential of the Tl Cu-site has been determined using potentiometric titrations with redox mediators for a large number of different laccases and varies between 410 mV vs. NHE for Rhus vernicifera [67] and 790 mV for laccases from Polyporus versicolor and Coriolus hirsutus [244,251]. The T2 and T3 sites have higher potentials [251]. 

Coriolus hirsutus (Wulf. Fr.) Quel. = Polyporus hirsutus Wulf. Fr. 

Laccase (Lacc) (300 - 500 U mg ) from Coriolus hirsutus was purified and supplied by the Research Center for Molecular Diagnostics, Moscow (Russia). Quinoprotein glucose dehydrogenase (GDH) (950 U mg - ) from Acinetobacter calcoaceticus, p-aminophenol (PAP) and p-aminophenol phosphate (PAPP) was kindly supplied by Boehringer Mannheim. All chemicals were used without further purification. 

Reductive biocatalytic transformations mediated by Cyt c have been exemplified in a variety of following systems. Cytc electrochemically reduced at a promoter-modified electrode surface has mediated the electron-transfer to soluble laccase (from Coriolus hirsutus), which biocatalyzed the reduction of O2 to water [320]. 

In an interesting work by Skladal, a four-electrode biosensor based on immobilization of laccase Coriolus hirsutus), peroxidase (horseradish) and t5Tosinase (mushroom) in a same screen-printed array was developed for generic monitoring of phenols [57]. The enzymes were immobilized onto a self-assembled monolayer (4-mercapto-l-butanol)-modified gold surface via covalent attachment by epichlo-rohydrin coupling. The experimental conditions for simultaneous operation of the... 

---