Thin-Layer Chromatography of Lipids

Padley, F.B., Thin-Layer Chromatography of Lipids, paper presented at the Thin-Layer Chromatography Proceedings Symposium, Rome 1963, 87 (published in 1964). 

Day 2 Thin-Layer Chromatography of Lipids, Quantitative Cholesterol Assay, Folin-Ciocalteau Assay, and Preparation for Phosphate Assay... 

Mangold, H. K. (1961) Thin layer chromatography of lipids. Journal of the American Oil Chemists Society, 38, 708-27. 

Morris, L. J. and Nichols, B. (1972) Argentation thin-layer chromatography of lipids, in Progress in Thin-layer Chromatography, Related Methods (ed. A. Niederwieser), Ann Arbor-Humphrey Science Publishers, Ann Arbor, MI, pp. 74-93. 

Shukla, V.K.S. (1995) Thin-layer chromatography of lipids, in New Trends in Lipid and Lipoprotein Analyses (eds J. L. Sebedio and E. G. Perkins), AOCS Press, Champaign, IL, pp. 17-23. 

II. Thin-Layer Chromatography of Lipids 1. Separation of Lipids According to Compound Class... 

Fried, B., and Sherma, J. (1990). Thin layer chromatography of lipids found in snails (Gastropoda Mollusca). J. Planar Chromatogr.—Mod. TLC 3 290-299. 

Kupke, I. R., and Zeugner, S. (1978). Quantitative high-performance thin-layer chromatography of lipids in plasma and liver homogenates after direct application of 0.5 pi samples to the silica gel layer. J. Chromatogr. 146 261-271. 

Figure 2 Thin layer chromatography of lipid acyl hydrolase activi in spinach leaves. In panel a, suspension of dried spinach leav were incubated at 30 for 0, 20, or 60 min with or without heai treatment (60 C) before applied onto a TLC plate. In panel b, the suspension was incubated at 30 X) for 0-18 hours. Triglyceride (TGC or T), diglyceride (DGC or D), monoglyceride (MGC or M), free fatty acids (FFA or F), and phosphatidylcholine (PC or P) were used as controls.

Brennan, P.J., M. Heifets, and B.P. Ullom Thin-layer Chromatography of Lipid Antigens as a Means of Identifying Nontuberculous Mycobacteria. J. Clin. Microbiol. 15, 447 (1982). 

Nakazawa, Y., Thin-layer chromatography of compound lipids, Kagaku No Ryoiki Zokan, 64,31,1964. 

Acid Treatment. This procedure, if applied to a sample containing all three species, would lead to release of a long-chain fatty aldehyde which could be isolated and analyzed as the dimethyl acetal. Subsequent thin-layer chromatography of the acid reaction mixture would allow recovery of the parent diacyl GPA and alkylacyl GPA. Then, using the chloroform soluble lipid phosphate as a guideline, base treatment of the original sample will lead to loss of chloroform-soluble phosphorus (representative of the diacyl GPA). The remaining chloroform-soluble phosphorus will represent the alkyl and alkenyl form. 

Dittmer, J. C. and Lester, R. L. (1964) A simiple specific spray for the detection of phospholipids on thin layer chromatography, J. Lipid Res. 5, 126-127. 

Gonzalez-Sastro, F. and Folch-Pi, J. (1968) Thin layer chromatography of the phos-phoinositides, J. Lipid Res. 9, 532-533. 

Ackman, R. G., Application of thin-layer chromatography to lipid separation, in Analysis of Fats, Oils and Lipoproteins (G. E. Perkins, ed.), American Oil Chemical Society, Champaign, IL, 1991, pp. 60-82. Christie, W. W., Lipid Analysis, 2nd ed., Pergamon Press, Oxford, 1982. 

Ackman, R.G. Application of Thin-Layer Chromatography to Lipid Separation. In Analysis of Fats, Oils and Lipoproteins Perkins, G.E., Ed. Amer. Oil Chem. Soc. Champaign, IL, 1991 60-82. 

The samples are collected (50 ml fractions) and their lipid composition is analyzed by thin layer chromatography. The lipids are stained by the use of methanolic sulfuric acid followed by an ashing process. The lipid is visible as dark spots. GDNT shows a Revalue of 0.45 (CHCI3 MeOH, 9 1, v v) in accordance to literature (0.45 (19) and 0.35 (20)). 

Skipsky, V.P., Peterson, R.F., Sanders, J., and Barclay, M. (1963) Thin-Layer Chromatography of Phospholipids Using Silica Gel Without Calcium Sulfate. J. Lipid Res. 4,227-228. 

Allan, D. and Cockroft, S. (1982) A modified procedure for thin-layer chromatography of phospholipids. Journal of Lipid Research, 23, 1373-4. 

Storry, J. E. and Tuckly, B. (1967) Thin-layer chromatography of plasma lipids by single development. Lipids, 2, 501-2. 

Fig. 1.- Thin layer chromatography of esterified cholesterol. Cholesterol esters were purified according to Horning et al. (I960) and run on TLC with heptane-ethyl ether (24 1). Spots were visualized with Liebermann-Bouchard reagent. Cholesterol oleate standard (St). Material from animals injected with Freund s adjuvant (FC) white matter (WM)j myelin (My) apoprotein of Folch s proteolipid (AFP) basic protein (BP) basic protein plus CNS lipids (BPL).

Raedsch, R., Hofmann, A. F., and Tsemg, K. (1979). Separation of individual sulfated bile acid conjugates as calcium complexes using reversed-phase partition thin-layer chromatography. J. Lipid Res. 20 796-800. 

Abramson, D., and M. Blecher Quantitative two-dimensional thin-layer chromatography of naturally occurring phospholipids. J. Lipid Res. 6, 628 (1964). 

Arvidson, G. a. E. Fractionation of naturally occuring lecithins according to degree of unsaturation by thin-layer chromatography. J. Lipid Res. 6, 574 (1965). 

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