Quantification electrophoretic mobility

High voltage electrophoresis (HVE) in agar gel with detection by bioautography has been used with considerable success in some laboratories for identification of residues (1-6). This procedure has the advantage that all antibiotic substances detectable by bioautography can be classified on the basis of electrophoretic mobility. Further testing may be required for quantification and to... 

CAE employing antibodies or antibody-related substances is currently referred to as immunoaf-hnity capillary electrophoresis (lACE), and is emerging as a powerful tool for the identification and characterization of biomolecules found in low abundance in complex matrices that can be used as biomarkers, which are essential for pharmaceutical and clinical research [166]. Besides the heterogeneous mode utilizing immobilized antibodies as described above, lACE can be performed in homogeneous format where both the analyte and the antibody are in a liquid phase. Two different approaches are available competitive and noncompetitive immunoassay. The noncompetitive immunoassay is performed by incubating the sample with a known excess of a labeled antibody prior to the separation by CE. The labeled antibodies that are bound to the analyte (the immuno-complex) are then separated from the nonbound labeled antibody on the basis of their different electrophoretic mobility. The quantification of the analyte is then performed on the basis of the peak area of the nonbonded antibody. 

Leukocyte proteases may be released if serum is allowed to sit on the clot too long after blood drawing. These proteases then form complexes with AAT, altering both electrophoretic mobility and in some cases immunochemical quantification. Bacterial contamination and release of... 

The most important issue in food quality analysis is food safety. Therefore, the development of rapid methods for the identification and quantification of bacterial contamination in foods is of utmost importance. Thus, a CE method with UV detection was proposed for the identification and quantification of bacterial contamination in food samples. The proposed method allowed for the effective separation of eight different types of bacteria in only 25 min. Electrophoretic resolution was improved by using cations in phosphate buffer (pH 7.0) that interacted with the bacterial surfaces changing its electrical properties and electrophoretic mobility. The validity of the method was established by comparison with the standard plate counting method, where bacterial cells were separated as... 

This aspect of /zTAS is considered by many to be one of the most important aspects permitting separation of complex analytical samples into separate components, eliminating the need for selective detection. While many different strategies have been reported, the two most common are based on electrophoresis and chromatography. In both cases, small volumes of sample are injected into a channel and the various components in the sample are separated on the basis of their electrophoretic mobility or their interaction with a stationary phase. The size of the sample plug will be vital to the reproducibility of the separation, both in terms of resolution and quantification. 

Native electrophoresis of pepsin and hemoglobin on 10% polyacrylamide gel carried out at 48 °C during 90 min, according to the Laemmli procedure, at pH 8.3 (Laemly 1970). Water solutions of all samples of enzyme (pepsin dissolved in water to final concentration of 2 mg/mL) were titrated with HCl to pH 2 and incubated at 37 °C, with addition of different concentrations of Al + ion (1, 5 and 10 mM). The samples were diluted with sample buffer in ratio 1 1 (v/ v) and applied on gel in volume of 20 pL. Visualization was performed with Commassie Brilliant Blue G-250 dye. The gels scanned and processed using Corel Draw 11.0 software package. Quantification of electrophoretic mobility of the molecule is carried out via Rs value, where it is defined by ... 

Quantification of electrophoretic mobility of the molecule is carried out via Rs value, where it is defined as ... 

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