Qualitative analysis high-performance liquid chromatography

Fan used a high performance liquid chromatographic method for the qualitative and quantitative analysis of miconazole [59], Miconazole sample was dissolved in methanol and determined by high performance liquid chromatography using methanol-water (75 25) as the mobile phase and ultraviolet detection at 214 nm, the recovery was more than 99.4% and the accuracy was satisfactory for the qualitative and quantitative analysis. 

Qualitative and quantitative analysis of bioactive principles in Zingiberis rhizoma by means of high performance liquid chromatography and gas liquid chromatography on the evaluation of Zingiberis rhizoma and chemical change of constituents. Yakugaku Zasshi 1993 113(4) 307-315. 

In earlier times, thin-layer chromatography (TLC), polyamide chromatography, and paper electrophoresis were the major separation techniques for phenolics. Of these methods, TLC is still the workhorse of flavonoid analysis. It is used as a rapid, simple, and versatile method for following polyphenolics in plant extracts and in fractionation work. However, the majority of published work now refers to qualitative and quantitative applications of high-performance liquid chromatography (HPLC) for analysis. Llavonoids can be separated. 

J.O. Doali A.A. Juhasz, Application of High-Performance Liquid Chromatography to the Qualitative Analysis of Compounds of Propellant and Explosives Interest , JChromatogrSci 12 (1), 51 -56 (1974) 92) F.T. Sweeney P.W.D. 

Enzyme immunoassay kits are now available for qualitative field testing or for laboratory screening and semiquantitative analysis of pesticides, herbicides, polychlorinated biphenyls (PCBs), mononuclear and polynuclear aromatic hydrocarbons, pentachlorophenol, nitroorganics, and many other compounds in aqueous and soil samples. Certain analytes may be quantitatively determined as well, with a degree of accuracy comparable to gas chromatography or high performance liquid chromatography determination. The method is rapid and inexpensive. 

Lant, M. S. Martin, L. E. Oxford, J. 1985. Qualitative and quantitative analysis of ranitidine and its metabolites by high-performance liquid chromatography-mass spectrometry. /. Chromatogr., 323,143-152. 

Another widely used method for qualitative and quantitative analysis of amino acid mixtures is high-performance liquid chromatography (HPLC) (see Experiments 2 and 6). The mixture of amino acids is first subjected to reaction with phenylisothiocyanate (PITC) to convert them to the phenylthiocarbamyl-amino acid derivatives, which are then subjected to chromatographic separation. The derivativatization of the amino acids serves two purposes it attaches a UV-absorbing tag, which makes their quantitative determination easy, and it converts them to a more hydrophobic form, which is necessary for good separation on the reverse-phase system commonly used with this technique. This method of amino acid analysis will be used in Experiment 6. 

High performance liquid chromatography (HPLC) has become one of the most powerful tools in contemporary organic analysis as the separation technique which can separate very complex mixtures of compounds and provide qualitative and quantitative information on the sample useful for the identification and determination of sample components. 

Before embarking on a study of high-performance liquid chromatography (HPLC), it is instructive to take a look at how this analytical technique emerged and to consider its relative importance in the field of analytical chemistry. Although HPLC is used to determine what is in a sample (qualitative analysis), its primary application is as a quantitative analytical tool and as such it plays a key role in analytical methods, since the analytical technique is only one part of the overall analytical method. An analytical method may be thought of as consisting of five distinct parts ... 

Other techniques, such as capillary electrophoresis and [matric-assisted laser desorption ionization] mass spectrometry, provide qualitative analyses, often with greater speed and sensitivity. Nevertheless, AAA by high-performance liquid chromatography (HPLC) complements other structural analysis techniques, such as peptide sequencing, and remains indispensable for quantifying proteinaceous materials. 

Analysis is an integral part of research, clinical, and industrial laboratory methodology. The determination of the components of a substance or the sample in question can be qualitative, quantitative, or both. Techniques that are available to the analyst for such determinations are abundant. In absorption spectroscopy, the molecular absorption properties of the analyte are measured with laboratory instruments that function as detectors. Those that provide absorbance readings over the ultraviolet-visible (UV-vis) light spectrum are commonly used in high-performance liquid chromatography (HPLC). The above method is sufficiently sensitive for quantitative analysis and it has a broader application than other modes of detection. 

The IMER approach does not require that the enzyme be placed in close proximity to the detector if the transducer signal is generated by a soluble product or cosubstrate of the enzymatic reaction. In the latter case, a variety of flow systems and postreactor detectors can be utilized to produce simultaneous determinations of the concentrations of several analytes. For example, an IMER can be combined with a high-performance liquid chromatography (HPLC) instrument (perhaps also in combination with mass spectroscopy) for purposes of both qualitative and quantitative analysis. The chemo-, stereo-, and regio-selectivities of enzymes facilitate separation and/or identification of analytes that may be present as different isomers (e.g., in peptide analysis based on use of peptidase IMERs in combination with these techniques to obtain structural information about the sequence of amino acids in peptides). 

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