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Pyridoxal phosphate structure

The principles of the above reactions form the basis of a series of important metabolic interconversions involving the coenzyme pyridoxal phosphate (structure 2.41). This condenses with amino acids to form a Schiff base (structure 2.42). The pyridine ring in the Schiff base acts as an electron sink which very effectively stabilizes a negative charge. [Pg.377]

This pyridoxal phosphate-requiring enzyme has been studied in several bacteria and X-ray crystal structures are available.35 The coryneform bacterium, Brevi-bacterium linens, is common on the surface of several cheeses, including Limburger and those of the Trappist type. The methionine y-lyase of this organism has been purified to homogeneity36 and the relevant gene, mgl (from MGL, abbreviation for methionine y-lyase) has been cloned and analyzed.37... [Pg.681]

Muscle glycogen phosphorylase is one of the most well studied enzymes and was also one of the first enzymes discovered to be controlled by reversible phosphorylation (by E.G. Krebs and E. Fischer in 1956). Phosphorylase is also controlled allosterically by ATP, AMP, glucose and glucose-6-phosphate. Structurally, muscle glycogen phosphorylase is similar to its hepatic isoenzyme counterpart composed of identical subunits each with a molecular mass of approximately 110 kDa. To achieve full activity, the enzyme requires the binding of one molecule of pyridoxal phosphate, the active form of vitamin B6, to each subunit. [Pg.238]

Neither Fj nor F2 alone gave the characteristic fluorescence of fa and nicked fa in the presence of L-serine and pyridoxal phosphate. However, titration of a fixed amount of F2 with F2 gave rise to a fluorescence intensity 80-90% that of nicked fa at a stoichiometric ratio of Ft to F2. Moreover, both the excitation and emission spectra of the stoichiometric mixture were the same as for nicked fa. In addition, the same specific quenching of this fluorescence was shown in recombined Fj and F2 as in nicked fa. Further, the dissociation constants for L-serine and for indole were determined to be the same within experimental error for recombined Fj and F2, as for nicked fa. No significant differences were found between nicked fa and reconstituted Fj F2 in the intrinsic fluorescence of the aromatic residues, or in the sedimentation coefficients or the 200-250 nm CD spectra. From the foregoing independent lines of evidence, F2 and F2 combine to produce a structure very similar to that of nicked fa. [Pg.83]

Lactobacillus delbrueckii. In 1953, Rodwell suggested that the histidine decarboxylase of Lactobacillus 30a was not dependent upon pyridoxal phosphate (11). Rodwell based his suggestion upon the fact that the organism lost its ability to decarboxylate ornithine but retained high histidine decarboxylase activity when grown in media deficient in pyridoxine. It was not until 1965 that E. E. Snell and coworkers (12) isolated the enzyme and showed that it was, indeed, free of pyridoxal phosphate. Further advances in characterization of the enzyme were made by Riley and Snell (13) and Recsei and Snell (14) who demonstrated the existence of a pyruvoyl residue and the participation of the pyruvoyl residue in histidine catalysis by forming a Schiff base intermediate in a manner similar to pyridoxal phosphate dependent enzymes. Recent studies by Hackert et al. (15) established the subunit structure of the enzyme which is similar to the subunit structure of a pyruvoyl decarboxylase of a Micrococcus species (16). [Pg.434]

Suicide Enzyme Inhibitors. Snicide substrates are irreversible enzyme inhibitors that bind covalently. The reactive anchoring group is catalytically activated by the enzyme itself through the enzyme-inhibitor complex. The enzyme thus produces its own inhibitor from an originally inactive compound, and is perceived to commit suicide. To design a substrate, the catalytic mechanism of the enzyme as well as the nature of the functional gronps at the enzyme active site must be known. Conversely, successful inhibition provides valuable information about the structure and mechanism of an enzyme. Componnds that form carbanions are especially usefnl in this regard. Pyridoxal phosphate-dependent enzymes form such carbanions readily becanse... [Pg.485]

At the same time, Snell and coworkers used model systems to achieve most of the reactions of the pyridoxal enzymes (Metzler and Snell, 1952a,b Olivard et al., 1952 Ikawa and Snell, 1954a,b Metzler et al 1954a,b Longnecker and Snell, 1957). They too developed the modern mechanisms for the series of reactions and demonstrated the role of the coenzyme as an electron sink by substituting alternative catalysts for pyridoxal phosphate. In particular, they showed that 2-hydroxy-4-nitrobenzaldehyde (Ikawa and Snell, 1954) functioned in their model systems just as did the vitamin its electronic structure is really quite similar (3). [Pg.6]

Vitamin B6 occurs naturally in three related forms pyridoxine (6.26 the alcohol form), pyridoxal (6.27 aldehyde) and pyridoxamine (6.28 amine). All are structurally related to pyridine. The active co-enzyme form of this vitamin is pyridoxal phosphate (PLP 6.29), which is a co-factor for transaminases which catalyse the transfer of amino groups (6.29). PLP is also important for amino acid decarboxylases and functions in the metabolism of glycogen and the synthesis of sphingolipids in the nervous system. In addition, PLP is involved in the formation of niacin from tryptophan (section 6.3.3) and in the initial synthesis of haem. [Pg.201]

Klrsch, J.F. Eliot, A.C. (2004) Pyridoxal phosphate enzymes mechanistic, structural and evolutionary considerations. Annu. [Pg.686]

T Although D-amino acids do not generally occur in proteins, they do serve some special functions in the structure of bacterial cell walls and peptide antibiotics. Bacterial peptidoglycans (see Fig. 20-23) contain both D-alanine and D-glutamate. D-Amino acids arise directly from the l isomers by the action of amino acid racemases, which have pyridoxal phosphate as cofactor (see Fig. 18-6). Amino acid racemization is uniquely important to bacterial metabolism, and enzymes such as... [Pg.858]

Species drawn in Appendix G are fully protonated. If a structure in Appendix G has a charge other than 0, it is not the structure that belongs with the name in the appendix. Names refer to neutral molecules. The neutral molecule pyridoxal phosphate is not the species drawn above, which has a +1 charge. The neutral molecule pyridoxal phosphate is... [Pg.162]

The vitamin biotin is formed in nature (left) by condensation of L-alanine with pimeloyl-CoAto form 8-amino-7-oxononanoate (AON). This compound is seen at the upper left of the center structure joined as a Schiff base with the coenzyme pyridoxal phosphate (PLP). This is a product complex of the enzyme AON synthase (see Webster et ah, Biochemistry 39,516-528,2000) Courtesy of D. Alexeev,... [Pg.718]

Compounds, often derivatives of vitamins that, while in the active site of the enzyme, alter the structure of a substrate in a way that permits it to react more readily. Coenzyme A, pyridoxal phosphate, thiamin diphosphate, and vitamin B12 coenzymes fall into this group. [Pg.719]

Figure 14-5 Some reactions of Schiff bases of pyridoxal phosphate, (a) Formation of the quinonoid intermediate, (b) elimination of a (3 substituent, and (c) transamination. The quinonoid-carbanionic intermediate can react in four ways (1—4) if enzyme specificity and substrate structure allow. Figure 14-5 Some reactions of Schiff bases of pyridoxal phosphate, (a) Formation of the quinonoid intermediate, (b) elimination of a (3 substituent, and (c) transamination. The quinonoid-carbanionic intermediate can react in four ways (1—4) if enzyme specificity and substrate structure allow.
Figure 14-6 Drawing showing pyridoxal phosphate (shaded) and some surrounding protein structure in the active site of cytosolic aspartate aminotransferase. This is the low pH form of the enzyme with an N-protonated Schiff base linkage of lysine 258 to the PLP. The tryptophan 140 ring lies in front of the coenzyme. Several protons, labeled Ha, Hb, and Hd are represented in NMR spectra by distinct resonances whose chemical shifts are sensitive to changes in the active site.169... Figure 14-6 Drawing showing pyridoxal phosphate (shaded) and some surrounding protein structure in the active site of cytosolic aspartate aminotransferase. This is the low pH form of the enzyme with an N-protonated Schiff base linkage of lysine 258 to the PLP. The tryptophan 140 ring lies in front of the coenzyme. Several protons, labeled Ha, Hb, and Hd are represented in NMR spectra by distinct resonances whose chemical shifts are sensitive to changes in the active site.169...
Observation of an abnormally large shift in the position of fluorescent emission of pyridoxal phosphate (PLP) in glycogen phosphorylase answered an interesting chemical question.187188 A 330 nm (30,300 cm ) absorption band could be interpreted either as arising from an adduct of some enzyme functional group with the Schiff base of PLP and a lysine side chain (structure A) or as a nonionic tautomer of a Schiff base in a hydrophobic environment (structure B, Eq. 23-24). For structure A, the fluorescent emission would be expected at a position similar to that of pyridoxamine. On the other hand, Schiff bases of the... [Pg.1295]

Figure 25-3 The structure of the two-enzyme a2p2 complex tryptophan synthase.65 66 The view is with the twofold axis of the OC2P2 complex vertical with the two a subunits at the ends and the P subunits in the center. The tunnel through which indole molecules released from indole propanol phosphate (IPP) in the a subunits to the pyridoxal phosphate (PLP) in the p subunits is shaded. Courtesy of C. Craig Hyde and Edith Wilson Miles. Figure 25-3 The structure of the two-enzyme a2p2 complex tryptophan synthase.65 66 The view is with the twofold axis of the OC2P2 complex vertical with the two a subunits at the ends and the P subunits in the center. The tunnel through which indole molecules released from indole propanol phosphate (IPP) in the a subunits to the pyridoxal phosphate (PLP) in the p subunits is shaded. Courtesy of C. Craig Hyde and Edith Wilson Miles.
As discussed in Chapter 2, section C2, pyridoxal phosphate condenses with amino acids to form a Schiff base (structure 8.44). Each of the three groups around the chiral carbon at the top of structure 8.44 may be cleaved to give an anion that is stabilized by delocalization of the electrons over the 7r orbitals. [Pg.471]

An essential feature of such stabilization is that the atoms in the tt system are planar. The extended molecular orbital is constructed from atomic orbitals that are perpendicular to the plane. Thus, for the electrons involved in any bond making or breaking processes to be stabilized by delocalization, the bonds that are being made or broken must also be perpendicular to the plane. This criterion may be used by pyridoxal phosphate-utilizing enzymes in choosing which bond jp cleave, as may be seen when the intermediate 8.44 is redrawn so that it is perpendicular to the plane of the paper (structures 8.45 the pyridine ring is represented as a solid bar). In each case, the bond that is broken is the one at the top, so that the electrons may be fed into the tt system. [Pg.471]

VITAMIN B (Pyridoxine). Infrequently called adermine or pyridoxol, this vitamin participates in protein, carbohydrate, and lipid metabolism. The metabolically active form of B6 is pyridoxal phosphate, the structures of which are ... [Pg.1700]

Structures of vitamin B6 derivatives and the bonds cleaved or formed by the action of pyridoxal phosphate (a). The reactive part of the coenzyme is shown in red in (a). The bonds shown in red in (d) are the types of bonds in substrates that are subject to cleavage. [Pg.201]

Structures of catalytic intermediates in pyridoxal-phosphate-dependent reactions. The initial aldimine intermediate resulting from Schiff s base formation between the coenzyme and the a-amino group of an amino acid (a). This aldimine is converted to the resonance-stabilized... [Pg.203]


See other pages where Pyridoxal phosphate structure is mentioned: [Pg.1313]    [Pg.408]    [Pg.553]    [Pg.50]    [Pg.165]    [Pg.107]    [Pg.206]    [Pg.312]    [Pg.83]    [Pg.49]    [Pg.154]    [Pg.7]    [Pg.256]    [Pg.1224]    [Pg.287]    [Pg.660]    [Pg.264]    [Pg.141]    [Pg.206]    [Pg.323]    [Pg.740]    [Pg.192]    [Pg.192]    [Pg.480]   
See also in sourсe #XX -- [ Pg.600 ]




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