Purkinje cells glutamate receptors

Bergersen, L., Waerhaug, O., Helm, J. et al. A novel postsyn-aptic density protein the monocarboxylate transporter MCT2 is co-localized with delta-glutamate receptors in postsynaptic densities of parallel fiber-Purkinje cell synapses. Exp. Brain Res. 136 523-34,2001. 

Okubo Y., Kakizawa S., Hirose K., and lino M. (2004). Cross talk between metabotropic and ionotropic glutamate receptor- mediated signaling in parallel fiber-induced inositol 1,4,5-trisphosphate production in cerebellar Purkinje cells. J. Neurosci. 24 9513-9520. 

However, presynaptic NMDA autoreceptors may also mediate opposite effects, i.e., a reduction of glutamate release. At parallel fiber Purkinje cell synapses, activation of presynaptic NMDA receptors caused significant reductions in excitatory postsynaptic currents (Casado et al. 2002). Likewise, spontaneous and evoked glutamate release from the nerve endings of primary afferents in the spinal cord was reduced when presynaptic NMDA receptors were activated (Bardoni et al. 2004). In contrast to this, substance P release at such synapses was shown to be enhanced by the activation of presynaptic NMDA receptors (Liu et al. 1997). 

The climbing fibers are now believed to use glutamate as transmitter, like the major proportion of mossy fibers. They are strongly enriched in glutamate (Ottersen et al., 1992) and their terminals are apposed to Purkinje cell thorns that exhibit a high density of AMPA receptors (Landsend et al., 1997). 

Hausser M, Roth A (1997) Dendritic and somatic glutamate receptor channels in rat cerebellar Purkinje cells. J... 

Batchelor AM, Madge DJ, Garthwaite J (1994) Synaptic activation of metabotropic glutamate receptors in the parallel fibre-Purkinje cell pathway in rat cerebellar slices. Neuroscience 63 9 1-915. 

Yuzaki M, Mikoshiba K (1992) Pharmacological and immunocytochemical characterization of metabotropic glutamate receptors in cultured Purkinje cells. J Neurosci 72 4253-4263. 

Araki K, Meguro H, Kushiya E, Takayama C, Inoue Y, Mishina M (1993) Selective expression of the glutamate receptor channel 82 subunit in cerebellar Purkinje cells, Biochem Biophys Res Commun 797 1267-1276. 

Hirano T, Kasono K, Araki K, Mishina M (1995) Suppression of LTD in cultured Purkinje cells deficient in the glutamate receptor delta 2 subunit. NeuroReporl 6 524-526. 

Zhao HM, Wenthold RJ, Wang YX, Petralia RS (1997) 8-Glutamate receptors are differentially distributed at parallel and climbing fiber synapses on Purkinje cells. J Neurochem 68 1041-1052. 

Fig. 6. Summary histogram of development of glutamate receptors at parallel [postnatal day 10 (PI0) to adult] and climbing (P2 to adult) fiber synapses on Purkinje cells of the cerebellum. Note especially the peak in immunogold labeling of the delta receptors at PI0-PI4 in climbing fiber synapses (cO, the peaks of the AMPA receptors (GluR2, GluR2/3 antibodies) at P2-P5, and the inverse patterns of peaks for parallel fiber synapses (pf) and climbing fiber synapses in adults for AMPA versus delta receptors. Modified from Zhao et al. (1998).

Kurihara H, Hashimoto K, Kano M, Takayama C, Sakimura K, Mishina M, Inoue Y, Watanabe M (1997) Impaired parallel fiber-Purkinje cell synapse stabilization during cerebellar development of mutant mice lacking the glutamate receptor 52 subunit. J Neurosci 77 9613-9623. 

Petralia RS, Zhao H-M, Wang Y-X, Wenthold RJ (1998) Variations in the tangential distribution of postsynaptic glutamate receptors in Purkinje cell parallel and climbing fiber synapses during development. Neuropharmacology 37 32l- 3M. 

Takayama C, Nakagawa S, Watanabe M, Mishina M, Inoue Y (1996) Developmental changes in expression and distribution of the glutamate receptor channel 82 subunit according to the Purkinje cell maturation. Dev Brain Res 92 147-155. 

Zhao H-M, Wenthold RJ, Petralia RS (1998) Glutamate receptor targeting to synaptic populations on Purkinje cells is developmentally regulated. J Neurosci 78 5517-5528. 

Galante M, Diana MA (2004) Group 1 metabotropic glutamate receptors inhibit GABA release at interneuron-Purkinje cell synapses through endocannabinoid production. J Neurosci 24 4865-4874... 

Pisu MB, Guioli S, Conforti E, Bernocchi G (2003) Signal molecules and receptors in the differential development of cerebellum lobules. Acute effects of cisplatin on nitric oxide and glutamate systems in Purkinje cell population. Brain Res Dev Brain Res 145 229-240. 

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