Pseudomonas luteola

Pseudomonas luteola Reactive azo dyes, Direct azo dyes and leather dyes The 59-99% color removal after 2-6 days static incubation, at dye concentration of 100 mg L 1, monoazo dyes showing fastest rate of decoloration [78]... 

Pseudomonas luteola, Eschericia coli Reactive Red 22 The E. coli improved the ability of Pseudomonas sp. to decolorize the dye by producing decolorization -stimulating extracellular metabolites [42]... 

Dye-containing wastewater and Reactive Red 22 Mixed cultures of Pseudomonas luteola and E. coli DH5a Presence of E. coli DH5a increased the decolorization efficiency of P. luteola even though DH5a was an inefficient decolorizer in this consortium [42, 95]... 

Chen BY, Chen SY, Lin MY, Chang JS (2006) Exploring bioaugmentation strategies for azodye decolorization using a mixed consortium of Pseudomonas luteola and Escherichia coli. Process Biochem 41 1574-1581... 

Hu TL (2001) Kinetics of azoreductase and assessment of toxicity of metabolic products from azo dyes by Pseudomonas luteola. Wat Sci Technol 43 261-269... 

Pseudomonas luteola Reactive red 22 Aerated flask Batch and fed-batch Aerobic growth, anaerobic decolorization F 200-4,000 28 n.a. 

Pseudomonas luteola Reactive red 22 Flask Sequential batch Static and agitated incubation E Ca arginate, k carageenan polyacryl. gel 0-200 20-47 5-10... 

Pseudomonas luteola Reactive red 22 Flask Batch Static incubation F - 0-400 20-47 5-9... 

Pseudomonas luteola, modified E.coli Reactive black b Flask Batch Aerobic growth, anaerobic conv. F 0-1,500 15-50 4-10... 

Pseudomonas luteola Reactive red 22 Fixed bed Continuous Anaerobic E Ca alginate, polyacrylamide gel 30-200 n.a n.a. 

Chang JS, Lin YC (2000) Fed-batch bioreactor strategies for microbial decolorization of azo dye using Pseudomonas Luteola strain. Biotechnol Progr 16 979-985... 

Chang JS, Chou C, Chen SY (2001) Decolorization of azo dyes with immobilized Pseudomonas luteola. Process Biochem 36 757-763... 

Chang JS, Chou C, Lin YC et al (2001) Kinetic characteristics of bacterial azo-dye decolorization by Pseudomonas Luteola. Water Res 35 2841-2850... 

Yeh MS, Chang JS (2004) Bacterial decolorization of an azo dye with a natural isolate of Pseudomonas luteola and genetically modified Escherichia coli. J Chem Technol Biotechnol... 

Lin HY, Leu JY (2008) Kinetic of reactive azo-dye decolorization by Pseudomonas Luteola in biological actived carbon process. Biochem Eng J 39 457 167... 

Metabolites formed during the decolourization of the azo dye Reactive red 22 by Pseudomonas luteola were separated and identified by HPLC-DAD and HPLC-MS. The chemical structures of Reactive red 22 (3-amino-4-methoxyphcnyl-/fhydroxyl-sulphonc sulphonic acid ester) and its decomposition products are shown in Fig. 3.92. RP-HPLC measurements were carried out in an ODS column using an isocratic elution of 50 per cent methanol, 0.4 per cent Na2HP04 and 49.6 per cent water. The flow rate was 0.5 ml/min, and intermediates were detected at 254 nm. The analytes of interest were collected and submitted to MS. RP-HPLC profiles of metabolites after various incubation periods are shown in Fig. 3.93. It was concluded from the chromatographic data that the decomposition process involves the breakdown of the azo bond resulting in two aromatic amines [154],... 

Fig. 3.92. A proposed mechanism for decolourization of Reactive red 22 by Pseudomonas luteola. (a) Chemical composition of Reactive red 22, (b), (c) products resulting from complete cleavage of the azo bond of Reactive red 22, (d) the product resulting from the partial reduction of the azo bond of reactive red 22. Reprinted with permission from J.-S. Chang et al. [154].

Fig. 3.93. The HPLC analysis on metabolites resulting from decolourization of reactive red 22 by Pseudomonas luteola (a) at the beginning of static incubation (IA = 3 639 667, /B = 130 140, Ic 116 243), (b) after static incubation for 4.7 h (/A = 2 231 542, /B = 230 559, Ic = 120 563), (c) after static incubation for 23.4 h (/A = 1 892 854, /B = 428 414, Ic = 205 169), (d) 3-amino t-methoxyphenyl /1-hydroxyl sulphone sulphonic acid ester (AMHSSAE), 90 per cent pure, 52 mg/1, and (e) products resulting from decolourization of Reactive red 22 by chemical reduction with SnCl2, (/A, /B, and 7C represent intensities of peaks A, B, and C, respectively). Reprinted with permission from J.-S. Chang et al. [154].

B. -Y. Chen, Understanding decolorization characteristics of reactive azo dyes by Pseudomonas luteola toxicity and kinetics. Proc. Biochem., 38 (2002) 437 446. 

Litthauer D, Ginster A, Skein EVE (2002) Pseudomonas luteola hpase a new member of the 320-residue Pseudomonas lipase family. Enzyme Microb Technol 30 209-215 Lopez-Serrano P, Cao L, van Rantwijk F et al. (2002) Cross-linked enzyme aggregates with enhanced activity application to lipases. Biotechnol Lett 24 1379-1383 Lorenz P, Liebeton K, Niehaus (2002) Screening for novel enzymes for biocatalytic processes accessing the metagenome as a resource of novel functional sequence space. Curr Opin Biotechnol 13 572-577... 

The characteristics of an extracellular mcl-PHA depolymerase purified from the marine isolate Pseudomonas luteola M13-4 have been reported by Rhee et al. (2006). This enzyme is similar to Pseudomonas alcaligenes LB 19 (Kim et al. 2002) but is different from poly(HB-co-HV) (PHBV) depolymerase of Xanthomonas sp JS02 (Kim et al. 2000a, b, c) the molecular mass is 28 kDa, the isoelectric point is 6.0 with a reactivity optimum at pH 10 and 40°C. Serine residues seem to play an important role in the hydrolysis reaction by analogy with P. alcaligenes LB 19. PHO has been totally degraded polyhydroxyhexanoate (PHH) and a copolymer of (PHB-co-60%HV) have been partially hydrolyzed. 

Rhee YA, Kim YH, SMn KS (2006) Characterization of an extracellular poly(3-hydroxyoctano-ate) depolymerase from the marine isolate Pseudomonas luteola Ml3-4. Enzyme Microb Technol 38 529-535... 

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