Alginate Pseudomonas

Pseudomonas luteola Reactive red 22 Fixed bed Continuous Anaerobic E Ca alginate, polyacrylamide gel 30-200 n.a n.a. 

A strain of Pseudomonas aeruginosa has been recently described, which shows the opposite enantioselectivity, converting racemic arylaminonitriles efficientiy into the D-amino acids. Again, whole-cell biocatalysis worked well, the cells being entrapped in alginate beads. It is unclear whether this biotransformation involves an amide intermediate. 

When cells of Pseudomonas sp. OS-K-29 immobilized on calcium alginate were incubated in 80 1 of a synthetic medium containing 0.2 vol.% of racemic 2,3-DCP, optically pure (S)-2,3-DCP of 100% e.e. was obtained. The immobilized cells could be re-used for a series of continuous reactions, a bioreactor being used for 19 reactions over 50 days without any loss of activity (Fig. 3). Optically pure (i )-2,3-DCP (100% e.e.) was also isolated from the racemate by means of stereospecific assimilation by Alcaligenes sp. DS-K-S38. Highly pure (R)- and (S)-EP of 99.5% e.e. were prepared from (S)- and (i )-2,3-DCP by treatment with aqueous NaOH, respectively [20-221 (Fig. 2). 

Ma, S. Wozniak, D.J. Ohman, D.E. Identification of the histidine protein kinase KinB in Pseudomonas aeruginosa and its phosphorylation of the alginate regulator algB. J. Biol. Chem., 272, 17952-17960 (1997)... 

A. Linker and R. S. Jones, A new polysaccharide resembling alginic acid isolated from Pseudomonas, J. Biol. Chem., 241 (1966) 3845-3851. 

Davies, D. G., A. M. Chakrabarty, and G. G. Geesey. 1993. Exopolysaccharide production in biofilms substratum activation of alginate gene expression by Pseudomonas aeruginosa. Applied and Environmental Microbiology 59 1181-1186. 

S Roychoudhury, NA Zielinski, AJ Ninfa, NE Allen, LN Jungheim, TI Nicas, AM Chakrabarty. Inhibitors of two-component signal transduction systems inhibition of alginate gene activation in Pseudomonas aeruginosa. Proc Natl Acad Sci (USA) 90 965-969, 1993. 

Alginate Pseudomonas aeruginosa Azotobacter vinelandii Range of viscosity types gels with Ca2 + textile printing, food applications... 

In Pseudomonas aeruginosa, a specific regulation of PolyP accumulation by the regulatory protein AlgR2 was revealed. This protein positively regulated the production of alginate,... 

H. Y. Kim, D. Schlictman, S. Shankar, Z. D. Xie, A. M. Chakrabarty and A. Komberg (1998). Alginate, inorganic polyphosphate, GTP and ppGpp synthesis co-regulated in Pseudomonas aeruginosa implications for stationary phase survival and synthesis of RNA/DNA precursors. Mol. Microbiol., 27, 717-725. 

Alginates are also produced extracellularly by some bacteria, such as Pseudomonas aeruginosa and Azotobacter vinelandii [100], where they are believed to play a role in biofilm formation, pathogenesis, and soil aggregation. Their gels, however, are inferior to seaweed alginates, because of the presence of 0-acetylation, which inhibits gel formation. 

Whole cells were employed [55] as the monitoring element in which Pseudomonas capacia capable of metabolizing aromatic compounds were immobilized in Ca+2-alginate beads and their response to aromatic substances, e.g., salicylate, was monitored with an enzyme thermistor. 

May TB, Shinabarger D, Maharaj R, et al. Alginate synthesis by Pseudomonas aeruginosa A key pathogenic factor in chronic pulmonary infections of cystic fibrosis patents. CUn Microbiol Rev 1991 4 191-206. 

Alginates were first described by Standford in 1881 [1]. They were originally discovered thanks to industrial developments related to iodine production. Alginates are quite widespread in Nature. They can be found as major cell-wall components (up to 45% of the dry matter) in marine brown algae (Phaeophyceae). They also occur in acetylated form as exopolysaccharides produced by bacteria belonging to Pseudomonas and Azotobacter genders. 

Biofiltration can be used to remove volatile organic compounds, including odors, from waste air.295 Pseudomonas spp. can remove toluene, and Bacillus spp., ethanol. The process is suitable for use on food-processing plants, public treatment works, and others, to control odors. The cost can be quite a bit lower than that of incineration or adsorption on carbon. A biofilter with Nitrosomonas eu ropaea immobilized in calcium alginate removed 97.5% of ammonia from air.296... 

Cyanovaleramide is used as intermediate for the synthesis of the DuPont herbicide azafenidine (Fig. 19-39). The whole cells from Pseudomonas chlororaphis are immobilized in calcium alginate beads. The biotransformation itself is catalyzed by a nitrile hydratase that converts a nitrile into the corresponding amide by addition of water. Nitrile hydratases belonging to the enzyme class of lyases (E.C. 4) are not be... 

Entrapment of Pseudomonas S-17 cells in calcium alginate on gold electrodes CO2 content in solutions [60]... 

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