Algins Pseudomonas aeruginosa

Alginate Pseudomonas aeruginosa Azotobacter vinelandii Range of viscosity types gels with Ca2 + textile printing, food applications... 

Alginate Pseudomonas aeruginosa Azotobacter sp. Polysaccharides with irregular structure composed of variable proportions of 1,4-linked -D-mannuronic acid (M) and its C-5 epimer a-L-guluronic (G) M blocks [-D-ManUA-(l —> G blocks [-l-GuIUA-(1 —> 4) a-]n Mixed block [DM(1 4)-LG-(l —> 4)-]n... 

A strain of Pseudomonas aeruginosa has been recently described, which shows the opposite enantioselectivity, converting racemic arylaminonitriles efficientiy into the D-amino acids. Again, whole-cell biocatalysis worked well, the cells being entrapped in alginate beads. It is unclear whether this biotransformation involves an amide intermediate. 

Ma, S. Wozniak, D.J. Ohman, D.E. Identification of the histidine protein kinase KinB in Pseudomonas aeruginosa and its phosphorylation of the alginate regulator algB. J. Biol. Chem., 272, 17952-17960 (1997)... 

Davies, D. G., A. M. Chakrabarty, and G. G. Geesey. 1993. Exopolysaccharide production in biofilms substratum activation of alginate gene expression by Pseudomonas aeruginosa. Applied and Environmental Microbiology 59 1181-1186. 

S Roychoudhury, NA Zielinski, AJ Ninfa, NE Allen, LN Jungheim, TI Nicas, AM Chakrabarty. Inhibitors of two-component signal transduction systems inhibition of alginate gene activation in Pseudomonas aeruginosa. Proc Natl Acad Sci (USA) 90 965-969, 1993. 

In Pseudomonas aeruginosa, a specific regulation of PolyP accumulation by the regulatory protein AlgR2 was revealed. This protein positively regulated the production of alginate,... 

H. Y. Kim, D. Schlictman, S. Shankar, Z. D. Xie, A. M. Chakrabarty and A. Komberg (1998). Alginate, inorganic polyphosphate, GTP and ppGpp synthesis co-regulated in Pseudomonas aeruginosa implications for stationary phase survival and synthesis of RNA/DNA precursors. Mol. Microbiol., 27, 717-725. 

Alginates are also produced extracellularly by some bacteria, such as Pseudomonas aeruginosa and Azotobacter vinelandii [100], where they are believed to play a role in biofilm formation, pathogenesis, and soil aggregation. Their gels, however, are inferior to seaweed alginates, because of the presence of 0-acetylation, which inhibits gel formation. 

May TB, Shinabarger D, Maharaj R, et al. Alginate synthesis by Pseudomonas aeruginosa A key pathogenic factor in chronic pulmonary infections of cystic fibrosis patents. CUn Microbiol Rev 1991 4 191-206. 

Figure 2 Pathway of alginate synthesis in Pseudomonas aeruginosa.

Leid, J. G., Willson, C. J., Shirtliff, M. E., Hassett, D. J., Parsek, M. R., and Jeffers, A. K. (2005). The exopolysaccharide alginate protects Pseudomonas aeruginosa biofilm bacteria from IFN-gamma mediated macrophage killing. J. Immunol. 175, 7512-7518. 

Pressler T, Pedersen SS, Espersen F, Hoiby N, Koch C. IgG subclass antibody responses to alginate from Pseudomonas aeruginosa in patients with cystic fibrosis and chronic P. aeruginosa infection. Pediatr Puhnonol 1992 14 44. 

Other water-soluble microbial polysaccharides include the anionic polysaccharides gellan from Pseudomonas elodea and bacterial alginate from Pseudomonas aeruginosa, and the neutral polysaccharides scleroglucan, curdlan, and pullulan (72,75,76,83). 

Damron FH, Napper J, Teter MA, et al. Lipotoxin F of Pseudomonas aeruginosa is an AlgU-dependent and alginate-independent outer membrane protein involved in resistance to oxidative stress and adhesion to A549 human lung epithelia. Microbiology. 2009 155 1028-38. doi 10.1099/mic.0.025833-0. 

Hentzer, M., Teitzel, G. M., Balzer, G. J., Heydorn, A., Molin, S., Givskov, M. and Parsek, M. R. 2001. Alginate overproduction affects Pseudomonas aeruginosa biofilm structure and function. Journal of Bacteriology 183, 5395-5401. 

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