Proteins micropatterning

Dosev D, Nichkova M, Liu MZ, Guo B, Liu GY, Hammock BD, Kennedy IM. Application of luminescent Eu Gd203 nanopar- 86. tides to the visualization of protein micropatterns. J. Biomed. 

Scott MA, Wissner-Gross ZD, Yanik ME (2012) Ultra-rapid laser protein micropattern-ing screening for directed polarization of single neurons. Lab Chip 12 2265-2276... 

A two-dimensional micropatterned tissue can be easily obtained by utihz-ing the inherent differences in cell adhesiveness between different micropatterned photografted regions. This was attained by photoiniferter graft polymerization with a projection mask placed on an iniferter-derivatized surface. Since protein adsorption and cell adhesion are markedly suppressed on nonionic graft polymers, such as polyDMAm, any anchorage-dependent cells such as endothelial cell adhere and proliferate only on nonirradiated surfaces, resulting in the formation of a two-dimensional patterned tissue or cellular sheet (Fig. 24). 

Mayer, M., et al. (2004) Micropatterned agarose gels for stamping arrays of proteins and gradients of proteins. Proteomics. 4, 2366-76. 

There are several recent examples of the switching of nonspecific protein binding on polymer surfaces by application of an external stimulus. Alexander and coworkers demonstrated that protein adhesion can be controlled on PNIPAM surface brushes [14, 181]. For instance, it was reported that the adsorption of FITC-labeled bovine serum albumin (FITC-BSA) on PNIPAM/hexadecanethiol micropatterned surfaces could be tuned by LCST. However, this effect was found to be less pronounced after prolonged incubation times or repeated heating/cooling cycles. The authors suggested that this behavior could be due to unspecific PNIPAM-protein interactions [14],... 

Keywords Assay Bioactive surface Cell adhesion Cell chip Hydrogel Microengineering Micropattern Peptide Polymer brush Protein adsorption... 

In an effort to identify the number of integrins required to trigger the formation of a stable FA, Spatz and co-workers studied a REF cell line on smaller nanostructured micropatterns. These experiments revealed that a minimum of six integrins per adhesive micropatterns were necessary to observe persistent cell adhesion. Adhesive islands with a side length larger than 1 pm induce the formation of FAs rich in paxillin, an integrin adaptor protein associated to actin fibres. These... 

Covalent immobilization of cytophilic proteins by iCP can be also used to pattern cells on substrates. Cytophilic proteins can be printed in micropatterns on top of reactive SAMs using, for example, imine chemistry.109 Aldehyde-functionalized... 

Protein patterning Trypsin Micropatterned sol-gel structures in polyd imethy 1 siloxane microchannels [368]... 

Britland S, Perez-Arnuad E, Clark P, McGinn B, Connolly P, Moores G. Micropatterning proteins and synthetic peptides on solid supports a novel application for microelectronics fabrication technology. Biotechnol. Prog. 1992 8 155-160. 

Bieri C, Ernst O P, Heyse S, et al. (1999). Micropatterned immobilization of a G protein-coupled receptor and direct detection of G protein activation. Nature Biotechnol. 17 1105-1108. 

Kim DK, Ganesan R, Jung CH, Hwang IT, Choi JH, Kim JB, et al. Micropatterning of proteins on ion beam-induced poly(acrylic acid)-grafted polyethylene film. Polym Adv Technol 2011 22(12) 1989-92. 

Yun JM, Jung CH, Kim DK, Hwang IT, Choi JH, Ganesan R, et al. Photosensitive polymer brushes grafted onto PTFE film surface for micropatterning of proteins. J Mater Chem 2010 20(10) 2007-12. 

Microfabrication enables the creation of complex tissue-engineered products that can be used to replace or augment failing organs and tissues. The number and variety of microfabricated tissue-engineered products have increased rapidly in the last several years microporous biocapsules to provide immunological protection to pancreatic islets micropatterned hepatocytes and mesenchymal cells as key components of liver assist devices microtextures basement-membrane-type structures to create skin with proper surface texture and microspatial control of the distribution of vitronection or other extracellular matrix proteins to promote the formation of mineralized bone-like tissue in vivo (Bhatia and Chen, 1999). 

Belu, A.M., Yang, Z., Asiami R Chilkoti, A. (2001) Enhanced TOF-SIMS imaging of a micropatterned protein by stable isotope protein labeling. Anal Chem., 73,143-150. 

A. (2009) Micropatterning of proteins and mammalian cells on indium tin oxide. ACS Appl. Mater. Interfaces, 1, 2592-2601. 

Patterned surfaces are widely used in 2D cell culture methods, where some receptor proteins or extracellular matrix are patterned on a surface for subsequent cell docking. Many microfabrication techniques described above can be used to form patterned surfaces. They allow a high degree of geometrical control over microscale features of a substrate. Micropatterned cell cultures are ideal to address fundamental issues of cell-cell and cell-surface interactions in confined geometries, as well as subcellular processes. 

In 2D cell culture on chip, cell adhesion has been extensively studied on patterned surfaces for it is critical to cellular functions. Micropatterns have been used to study the cellular interactions with various materials such as metals, polymers, self-assembled monolayers, extracellular matrix proteins, cell-adhesion peptides, and other bioactive molecules. The physical and chemical proper-... 

Recently, the interest on adhesive fibrillar surfaces has turned to include adhesion against soft substrates or under wet environments. This is achieved by coating the micropatterned substrates with wet adhesives (Figure 15.9) [43—45] or viscous oils [46]. Patterned PDMS [44] or PUR [45] surfaces were coated with poly(dopamine methacrylate-co-2-methoxyethyl acrylate), a copolymer containing high amounts of cathecol, and a key component of wet adhesive proteins fotmd in mussels. Such... 

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