Proteins beef flavor

Food-derived anticarcinogens, conjugated dienoic derivatives of linoleic acid, 262-270 Food quality health concerns, 5-6 maintenance by natural and synthetic compounds, 56-76 molecular s proaches to study, 1-6 related to antimicrobial peptides, 303-318 Foodbome pathogens, ionizing radiation, 296-298 Free radical(s) beef flavor peptides, 89-91 oxidation generating system, 88-89,91/ protein composition, 88-89,91/... 

Borden Industrial Food Products, Northbrook, Illinois, manufacture Wyler Soups and Wyler Brand CB-M flavor concentrates. One of the latter, for example, 78-62 Beef Flavor, contains hydrolyzed vegetable protein, dextrose, sucrose, vegetable oil, salt, monosodium glutamate, disodium inosinate, disodium guanylate, onion powder, and garlic powder. They are similar to, but not identical with, Pfizer s CORRAL, which also contains arabinose, cysteine, P-alanine, and glycine. Wyler Brand 78-50 Chicken Flavor also contains some chicken. 

The structure of the food matrix is also known to affect the release of volatile compounds having an impact on flavors and aroma. Changes in flavor result from the interactions of lipid-derived carbonyl compounds by aldolization with the amino groups of proteins. Undesirable flavors are produced when beef or chicken are fried in oxidized fats by the interaction of secondary lipid oxidation... 

Soy proteins are used extensively in meat and meat products by the military, the school lunch program and consumers to save money. Their ultimate acceptability is equally dependent upon the nutritional, chemical, sensory and shelf life changes which occur when they are added. Soy proteins in meat products such as ground beef inhibit rancidity, improve tenderness, increase moisture retention, decrease cooking shrink, fat dispersion during cooking and have no important effect on microbiological condition. Concomittantly, inordinate amounts of added soy protein may cause the meat product to be too soft, exhibit an undesirable flavor and may lead to a decreased PER and a deficiency in B-vitamins and trace minerals. In emulsified meat products, soy protein effectively binds water but does not emulsify fat as well as salt soluble muscle protein. Prudent incorporation of plant proteins can result in an improvement of the quality of the meat product with inconsequential adverse effects. 

When structured soy protein fiber was added to fermented salami at 15 or 30% levels, trained sensory panels found the flavor to be undesirable, whereas a 116-member untrained panel found the product containing 30% soy flour to be undesirable in flavor, tenderness and overall desirability (26). The flavor of beef patties containing 20% soy protein flour or concentrate was rated about equal to all beef patties by a 52-member panel, whereas patties containing 30% were scored lower by the panel (6). Berry et al. (7) found the characteristic "soy-like" flavor to be more... 

Textured Soy Proteins. Textured vegetable proteins, primarily textured flours and concentrates (50% protein and 70% protein, dry basis, respectfully) are widely used in the processed meat industry to provide meat-like structure and reduce ingredient costs (3-6, 9-10). Available in a variety of sizes, shapes, colored or uncolored, flavored or unflavored, fortified or unfortified, textured soy proteins can resemble any basic meat ingredient. Beef, pork, seafood and poultry applications are possible 03, 4-7, 15, 19) Proper protein selection and hydration is critical to achieving superior finished product quality. Textured proteins have virtually no solubility and, thus, no ability to penetrate into whole muscle tissue Therefore, textured soy proteins are inherently restricted to coarse ground (e.g. sausage) or fine emulsion (e.g. weiners and bologna) products, and comminuted and reformed (i.e. restructured) meat products. None are used in whole muscle absorption or injection applications (2-4, 6, 11). 

The flavor enhancer monosodium glutamate (MSG) is currently used in virtually every type of savory prepared-food. Unfortunately, MSG has several deleterious side effects on a large proportion of the population. Fortunately, a naturally occurring peptide isolated from a muscle food (beef) can serve not only as a potential replacement for MSG but also as a nutritional adjuvant. The peptide, called BMP or beefy meaty peptide, acts as a flavor enhancer and is found to occur naturally in beef Chapter 6). Research on BMP suggest that it is not only non-allergenic but, by virtue of its protein composition, is a nutritionally sound replacement for MSG. 

Figure 6. Multivariate factor analysis of beef showing the " distribution" of experimental treatments, volatile chemical attributes, chemical protein elements and flavor attributes in beef samples during postmortem aging. Insert represents the data redrawn with the graphical origin depicted as the fulcrum of a balance beam.

The changes in the protein composition and flavor of "cooked" and "cooked- -stored" beef seemed to be related to degradation by free radical species induced during lipid oxidation 1-10, 16, 22, 23), Based on the information presented in the aforementioned publication, it seemed reasonable to suggest that the appearance of "bitter" and "sour" tastes and the disappearance of "meaty" and "beefy" flavors were a result of the activity of the free radicals derived from lipid oxidation on flavor proteins (2, 24-28),... 

Table I summarizes the various meats, meat constituents, and other related substances which have been analyzed, including substances reported on previously (6) as well as those for which new data are given. The substances chosen are intended to provide a cross-section of the type of inherently related material from which volatile irradiation odor and flavor compounds might be expected to form. Thus, in addition to several whole meats, the volatile irradiation products from a number of protein and lipid substances have been analyzed. Among the lipid substances included are typical whole fats and separate moieties such as triglycerides, fatty acid esters, and cholesterol, as an example of a steroid. Among the proteinaceous substances included are a protein, a polypeptide, and some individual amino acids. Finally, beef itself has been separated into a protein, a lipid, and a lipoprotein fraction, and these have been separated, irradiated, and analyzed.

Merritt (42) has carefully studied the yields of hydrocarbons on irradiation of meat and meat components and proposed mechanisms for their formation during irradiation. Despite this recent progress, the chemical characterization of irradiation flavor in meats is far from complete. Little is known about the radiation-induced chemical processes giving rise to the compounds proposed as important to irradiation flavor or the identity of the chemical precursors of these compounds. However, irradiation flavor in beef appears to be associated largely with the protein constituents in meat (21). 

Concentrations of thermally generated meat flavor components are diminished by protein adsorption when soy extenders are added to fresh meat products before heating. The amounts of individual alkyl pyrazines, thermally generated by heating beef diffusate, decreased linearly as the amount of whole soy, soy 7S or soy 11S proteins were increased in a model system. Similar recoveries were obtained when pyrazines were mixed with soy either as chemical standards or from diffusate. Stoichiometry and energetics of interaction were determined for methyl pyrazine congeners with soy proteins at 120° and 145°C. Results of this study suggest that flavorants can be added in readily determined amounts to compensate for losses due to adsorption in meat-soy products. 

In this study the physical parameters involved in interaction of a major class of meat flavorants, methyl pyrazines, with soy proteins were determined at meat roasting temperatures. Beef diffusate, the water soluble, low molecular weight fraction that constitutes about IX of beef, was shown to contain the necessary precursors to obtain a desirable, thermally generated meat aroma (8). Diffusate was heated under controlled conditions and generated volatiles were transferred to a gas chromatograph for separation and quantitation. Methyl pyrazines, either from heated diffusate or from standard solutions, were measured in the presence of purified soy proteins and the thermodynamics of binding were determined. 

Malonaldehyde, a major product of autoxidatlon of polyunsaturated fatty acids is a very reactive substance and reacts with amino acids, proteins and other chemical substances present in meats. Its concentration is generally determined by the 2-thlobarblturlc acid (TEA) test. Malonaldehyde may be used as an indicator for evaluation of the oxidative state of cooked meats. It has been reported that warmed-over flavor in beef is generally perceived when TEA number of cooked meats exceed numerical values of 0.5 to 1.0 (31). Malonaldehyde has also been implicated as having mutagenic and perhaps carcinogenic effects (32). Its presence further affects the rheological properties and texture of cooked meat products. Despite these, malonaldehyde has very little or no... 

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