CaMV promoters

Human hemoglobin (< + P) CaMVenhanced 35S promoter/ CaMV 35S terminator Transit peptide of small subunit of RubisCO of Pisum sativum N. tabacum 0.05% of seed protein 37... [Pg.94]

P-Interferon CaMV 35S promoter/ CaMV 35S terminator Native LP N. tabacum 170 ng g 1 FW 49... [Pg.97]

Dog gastric lipase CaMV enhanced 35S promoter/ CaMV 35S terminator LP of rabbit gastric lipase precursor LP of sweet potato sporamin precursor N. tabacum (leaves secretion) N. tabacum (leaves vacuole) 7% of acid extractable proteins 5% of acid extractable proteins 51... [Pg.97]

CaMV 35S promoter a promoter sequence from the CaMV... [Pg.655]

The first hurdle encountered during the development of alfalfa as a recombinant protein production system was the relative inefficiency of the available expression cassettes. A study in which a tomato proteinase inhibitor I transgene was expressed in tobacco and alfalfa under the control of the cauliflower mosaic virus (CaMV) 35S promoter showed that 3-4 times more protein accumulated in tobacco leaves compared to alfalfa leaves [5]. Despite the low efficiency of the CaMV 35S promoter in alfalfa, bio-pharmaceutical production using this system has been reported in the scientific literature. Such reports include expression of the foot and mouth disease virus antigen [6], an enzyme to improve phosphorus utilization [7] and the anti-human IgG C5-1 [8]. In this last work, the C5-1 antibody accumulated to 1% total soluble protein [8]. [Pg.4]

Fig. 1.1 Promoter activity in alfalfa leaves. Accumulation ofp-glucuroni-dase achieved in transgenic alfalfa leaves expressing the gusA gene under the control of CaMV 35S and alfalfa promoters. %TSP, percentage of total soluble proteins.

As indicated in Table 2.1, most of the promoters used in plant tissue culture have been based on the constitutive cauliflower mosaic virus (CaMV) 35S promoter. In contrast, inducible promoters have the advantage of allowing foreign proteins to be expressed at a time that is most conducive to protein accumulation and stability. Although a considerable number of inducible promoters has been developed and used in plant culture applications, e.g. [32-37], the only one to be applied thus far for the production of biopharmaceutical proteins is the rice a-amylase promoter. This promoter controls the production of an a-amylase isozyme that is one of the most abundant proteins secreted from cultured rice cells after sucrose starvation. The rice a-amylase promoter has been used for expression of hGM-CSF [10], aranti-trypsin [12, 29, 38, 39] and human lysozyme [30]. [Pg.25]

EGF (synthetic gene) CaMV 35S promoter/ nos terminator Codon-optimized for high level peptide expression in Escherichia coli N. tabacum (leaves) 0.001% ofTSP 38... [Pg.94]

Isoform ofhGAD (hGAD65) CaMV 35S promoter/ nos terminator GAD67i-87(rat)/hGAD65s8-58s Fusion Expression mediated by potato virus X infection N. tabacum (leaves) Daucus carota (tap root) N. tabacum (leaves) N. benthamiana (leaves) 0.01-0.04% ofTSP 0.01% ofTSP 0.04% ofTSP 0.19% ofTSP 2.2% ofTSP 39 40... [Pg.94]

Human collagen CaMV 35S promoter/ Pathogenesis-related protein (PR) LP or tobacco > 100 4g g 1 tissue 41... [Pg.95]

Binding subunit CaMV 35S promoter/TEV With and without KDEL N. tabacum 5 fig g-1 TSP 44... [Pg.95]

Cholera toxin B subunit CaMV 35S promoter/ nos terminator Kozak sequence, KDEL Lycopersicon esculentum Leaves 0.02% ofTSP Fruits 0.04% ofTSP 45... [Pg.96]

IL-10 CaMV 35S promoter/ nos terminator Native LP, with and without KDEL N. tabacum 800 ng g-1 TSP (without KDEL) 55ngg 1TSP (with KDEL) 13... [Pg.96]

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