Primordial germ cells culture

Godin, I., Deed, R., Cooke, J., Zsebo, K., Dexter, M., and Wylie, C. C. (1991). Effects of steel gene product on mouse primordial germ cells in culture. Nature 352 807-809. 

Matsui, Y Toksoz, D., Nishikawa, S., Nishikawa, S-I., Williams, D Zsebo, K., and Hogan, B. L. M. (1991). Effect of Steel factor and leukaemia inhibitory factor on murine primordial germ cells in culture. Nature 353 750-752. 

Resnik, J. L., Bixler, L. S., Cheng, L., and Donovan, P. J. (1992). Long-term proliferation of mouse primordial germ cells in culture. Nature 359 550-551. 

Shamblott MJ, Axelman J, Wang S, Bugg EM, Littlefield JW, Donovan PJ, Blumenthal PK, Huggins GR, Gearhart JD. Derivation of pluripotent stem cells form cultured human primordial germ cells. Proc Nat Acad Sci USA 1998 95 13726-31. 

Human embryonic stem cells were first collected in 1998 by two different research teams. The cells obtained from the inner cell mass of the blastocyst (4- to 5-day embryo) are embryonic stem cells (ESC) in contrast, cells cultured from the primordial germ cells of 5- to 9-week fetuses are embryonic germ cells (EGC). In the laboratory, ES or EG cells can proliferate indefinitely in an undifferentiated state but can also be manipulated to become specialized or partially specialized cell types, a process known as directed differentiation. Both ES and EG cells are pluripotent, meaning they have the potential to develop into more than 200 different known cell types. This class of human stem cells holds the promise of being able to repair or replace cells or tissues that are damaged or destroyed by many of our most devastating diseases and disabilities. 

K. J. Shamlott et al., Derivation of pluripotent stem cells from cultured human primordial germ cells , Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 13726-13731. 

Shim H, Gutierrez-Adan A, Chen LR, BonDurant RH, Behboodi E, Anderson GB (1997) Isolation of pluripotent stem cells from cultured porcine primordial germ cells. Biol Rq>rod 57(5) 1089-1095. 

Dolci S, WilliamsDE, Ernst MK, Resnick JL, Brannan Cl, Lock LF, Lyman SD, Boswell HS, Donovan PJ (1991) Requirement for mast ceU growth factor for primordial germ cell survival in culture. Nature. 352(6338) 809-811. 

Kawase E, Yamamoto H, Hashimoto K, Nakatsuji N (1994) Tumor necrosis factor-alpha (TNF-alpha) stimulates proliferation of mouse primordial germ cells in culture. Dev Biol 161(l) 91-95. 

Matsui Y, Zsebo K, Hogan BL (1992) Derivation of pluripotential embryonic stan cells from murine primordial germ cells in culture. Cell 70(5) 841-847. 

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