Polyvinyl alcohol electrophoresis

Effect of adsorbed polymer on the double-layer. Because of the presence of adsorbed train segments, the double layer is modified. The zeta-potential, , is displaced because the adsorbed polymer displaces the plane of shear. The parameters for describing adsorbed polymers are the fraction of the first layer covered by segments, 0, and the effective thickness, A, of the polymer layer, The insert gives the distribution of segments over trains and loops for polyvinyl alcohol adsorbed on silver iodide. Results obtained from double layer and electrophoresis measurements. 

Electrophoresis protein separation membranes have been prepared by step-growth condensation of water-soluble polyvinyl alcohol with selected water-soluble difunctional crosslinking agents. These membranes have broad pore size ranges, restricted pore size distribution, greater resistance to hydrolysis in an alkaline medium, and improved gel clarity when higher amounts of crosslinkers are used. 

TABLE 2. Experimental electrophoresis protein separation conditions using membranes prepared by reacting polyvinyl alcohol having a M of roughly 20,000 daltons 97.5% to 99.5% hydrolyzed and selected crosslinking agents. 

Possible Effect of Charged End-groups. A possible reason for differences between samples might be the process used for polymerising the original polyvinyl acetate. Emulsion polymerisation is likely to introduce a proportion of ionic (sulphate or carboxyl) end-groups which would not be expected if bulk polymerisation with benzoyl peroxide had been used. An Antweller Microelectrophoresis apparatus was used to measure rates of electrophoresis of polyvinyl alcohols in solution in a pH 7.8 phosphate buffer. No significant difference was observed between... 

Mori, M., Kaseda, M., Yamamoto, T., Yamada, S., and Itabashi, H. (2012) Capillary ion electrophoresis-capacitively coupled contactless conductivity detection of inorganic cations in human saliva on a polyvinyl alcohol-coated capillary. Anal. Bioanal. Chem., 402 (7), 2425-2430. 

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