Polysaccharides spectrometry

During the past decade, MALDI-TOF MS has proven to be an effective tool for the analysis of oligo- and polymeric mannoglucans (for extensive reviews see [222,223]). SEC/MALDI mass spectrometry was employed in the analysis of hemicelluloses isolated by microwave heat-fractionation from spruce and aspen wood [94]. These methods allowed the separation and characterization of the oligo- and polysaccharide fractions derived from the xylan and mannan components of both woods [224]. 

Qian, W.J., Jacobs, J.M., Camp, D.G., 2nd, Monroe, M.E., Moore, R.J., Gritsenko, M.A., Calvano, S.E., Lowry, S.F.,Xiao, W.,Moldawer, L.L., Davis, R.W., Tompkins, R.G., Smith, R.D. (2005a). Comparative proteome analyses of human plasma following in vivo lipo-polysaccharide administration using multidimensional separations coupled with tandem mass spectrometry. Proteomics 5, 572-584. 

Hell eur, R.J. Thibault, P. Optimization of Pyrolysis-Desorption CI-MS and Tandem Mass Spectrometry of Polysaccharides. Can. J. Chem. 1994, 72, 345-351. 

However, two other applications of mass spectrometry to carbohydrate chemistry seem the most important and promising. These are the identification of partially methylated monosaccharides, and structural analysis of di- and oligo-saccharides (and penetration of this new technique into polysaccharide chemistry). 

However, the data available suggest that the results obtainable from mass spectrometry will considerably facilitate the structural analysis of oligosaccharides and thus permit direct investigation of the products of partial hydrolysis of polysaccharides on the oligomer level. 

A. Gamian, E. Romanowska, A. Romanowska, C. Lugowski, J. Dabrowski, and K. Trauner, Citrobacter lipopolysaccharides Structure elucidation of the O-specific polysaccharide from strain PCM 1487 by mass spectrometry, one-dimensional and two-dimensional H NMR spectroscopy and methylation analysis, Eur. J. Biochem., 146 (1985) 641-647. 

Schulten, H.-R., and Gortz, W. (1978). Curie-point pyrolysis and field ionization mass spectrometry of polysaccharides. Anal. Chem. 50,428-433. 

Adsorption of Proteins and Polysaccharides at Aqueous—Solid Interfaces by Infrared Internal Reflection Spectrometry In Situ Investigation... 

Infrared-spectrometry (IR) was used for the investigation of substituent acetyl groups in the molecules of the polysaccharides. A Fourier transform IR Perkin-Elmer spectrometer, Model 1720-X, was used. The... 

Lupescu, N., Solo-Kwan, J., Christiaen, D., Morvan, H., and Arad, S. M. (1992). Structural determination by means of gas chromatography-mass spectrometry of 3-0-(a-D-gluco-pyranosyluronic acid)-galactopyranose, an aldobiuronic acid derived from Porphyridium sp. polysaccharide. Carbohydr. Polym. 19 131-134. 

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