Polymerase chain reaction , random

Differential display is a method for identifying differentially expressed genes, using anchored oligo-dT, random oligonucleotide primers and polymerase chain reaction on reverse-transcribed RNA from different cell populations. The amplified complementary DNAs are displayed and comparisons are drawn between the different cell populations. 

Leung, D.W., Chen, E. and Goeddel, D.V. (1989) A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction. Technique, 1, 11-15. 

Guo, Z.G. and Johnson, A.M. (1995) Genetic characterization of Toxoplasma gondii strains by random amplified polymorphic DNA polymerase chain reaction. Parasitology 111, 127-132. 

Joachim, A., Daugschies, A., Christensen, C.M., Bj0rn, H. and Nansen, P. (1997) Use of random amplified polymorphic DNA-polymerase chain reaction... 

Wu, Z., Nagano, I. and Takahashi, Y. (1998) The detection of Trichinella with polymerase chain reaction (PCR) primers constructed using sequences of random amplified polymorphic DNA (RAPD) or sequences of complementary DNA encoding excretory-secretory (E-S) glycoproteins. Parasitology 117, 173-183. 

List of Abbreviations GABA, gamma-aminobutyric acid type A 5HT3, 5-hydroxytryptamine type 3 SDM, site-directed mutagenesis PCR, polymerase chain reaction TRCP, targeted random chimera production SDS, sodium dodecyl sulphate... 

Ellington and Szostak365 synthesized a random "pool" of 1015 different oligodeoxyribonucleotides, each -100 nucleotides in length. They "amplified" these using the polymerase chain reaction (PCR Section H,6) and prepared a mixture of the corresponding RNAs by in vitro transcription. From the 1013 different sequences still present they selected individual... 

In addition to natural ribozymes several synthetic ribozymes with new activity have been obtained by in-vitro selection techniques. Starting with a pool of random RNA sequences, molecules with a desired activity can be isolated by successive cycles of activity selection, reverse transcription into DNA, and amplification by polymerase chain reaction [4a,b]. 

Fromant, M., Blanquet, S., and Plateau, P. (1995). Direct random mutagenesis of genesized DNA fragments using polymerase chain reaction. Analytical Biochemistry 224, 347-353. 

Random amplification of polymorphic DNA (RAPD) is an in vitro amplification technique (see section on polymerase chain reaction below) that eliminates the need for any prior characterization of the genome to be analyzed.12 14 Based on the random amplification of DNA segments using single short primers of arbitrary sequence, RAPD readily detects genetic polymorphisms in most organisms. The method is simple and rapid to... 

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