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Polyclonal antibodies, comparison

A. nidulans with selected phages of classes A, B, C, D and E resulted in increased polygalacturonase activity in comparison with the wild type strain. The culture media of pga transformants were further analysed by Western blotting with polyclonal antibodies raised against PGI and in all samples examined cross-reactive bands with molecular masses similar to that found for PGI or PGII were detected. [Pg.826]

Annexins from liver plasma membranes, monospecific polyclonal antibodies Purification, comparison with cellulose fiber modules Anion Exchange disks, Affinity (Protein A,Protein G), Affinity (annexins) [73]... [Pg.75]

Hsu, S. M., Cossman, J. C., and Jaffe, E. S. (1983) A comparison of ABC, unlabeled antibody and conjugated antibody methods with monoclonal and polyclonal antibodies—an examination of germinal center of tonsils. Am. J. Clin. Pathol. 80, 429 35. [Pg.222]

Anderson, C., Rezuke, W. N., Kosciol, C. M., Pasmszak, W. T., and Caitun, R. W. (1991) Identification of T-cell lymphomas in paraffn-embedded tissues using polyclonal anti-CD3 antibody comparison with frozen section immunopheno-typing and genotypic analysis. Mod. Pathol. 4, 358-362. [Pg.437]

Siemann, M., Syldatk, C. and Wagner, F. (1993) Detection and comparison of strains with selective L-hydantoin cleaving activity using polyclonal antibodies. Biotechnol. [Pg.242]

A polyclonal antibody, which by nature cannot be monospecific, can, if its titer is very high, behave like a monospecific antibody in comparison with the low titers of irrelevant antibodies in the same serum. Thus, this method is faster and performs better than other polyclonal antibody techniques while retaining all the advantages of polyclonal antibodies. [Pg.6]

Sunahara, N., Kawata, S., Kaibe, K., Furuta, R., Yamayoshi, M., Yamada, M., and Kurooka, S., Differential determination of recombinant human interleukin-1 alpha and its deamidated derivative by two sandwich enzyme immunoassays using monoclonal antibodies. Comparison with a polyclonal antibody-based competitive enzyme immunoassay. J. Immunol. Meth. 119, 75-82... [Pg.43]

P3. Porstmann, T., Porstmann, B., Nugel, E., Micheel, B., Karsten, U., and Fiebach, H., Comparison of monoclonal and polyclonal antibodies in a two-site binding enzyme immunoassay for alphafetoprotein (AFP). Clin. Chim. Acta 135, 13-22 (1983). [Pg.108]

TABLE 2 Comparison of Methods for the Adsorption of HSA on Anti-HSA Immunoadsorbents with Various Densities of Immobilized Polyclonal Antibodies Flow Rate 8.3 mm s"1... [Pg.365]

Several authors have compared imprinted polymers to biological receptors and even the term plastic antibodies has been coined to describe these remarkable materials. To a large extent, this comparison rests on a spectacular work of Mosbach and co-workers [24], who showed that theophylline- and diazepam-imprinted polymers displayed a specificity rather similar to that of polyclonal antibodies in binding studies with the template and its close structural analogues. However, it is the recognition of nucleotide or oligopeptide sequences which is... [Pg.208]

Tracy RP, Andrianorivo A, Riggs BL, Mann KG. Comparison of monoclonal and polyclonal antibody-based immunoassays for osteocalcin a study of sources of variation in assay results. J Bone Miner Res 1990 5 451-61. [Pg.1963]

To my knowledge there exists a single report describing the direct comparison of the relative effectiveness of monoclonal and polyclonal antibodies in the... [Pg.207]

Comparison of some properties of monoclonal and polyclonal antibodies... [Pg.61]

Thompson NE, Pattee PA (1977) Transformation in Staphylococcus aureus role of bacteriophage and incidence of competence among strains. J Bacteriol 129 778-788 Thompson NE, Razdan M, Kuntsmann G, Aschenbach JM, Evenson ML, BergdoU MS (1986) Detection of staphylococcal enterotoxins by enzyme-Unked immunosorbent assays and radioimmunoassays comparison of monoclonal and polyclonal antibody systems. Appl Environ Microbiol 51 885-890... [Pg.181]

Sharkey, R. M., Primus, F. J., Shochat, D., and Goldenberg, D. M. (1988) Comparison of tumour targeting of mouse monoclonal and goat polyclonal antibodies to carcinoembryonic antigen in the GW-39 human tumour-hamster host model. Cancer Res. 48, 1823-1828... [Pg.109]

The technique described above was used to characterize several monoclonal (14) as well as recombinant (15) antibodies. In the case of polyclonal antibodies, exact kinetic parameters are not obtained (Note 8) nevertheless, the quantitative comparison of different products is possible. Moreover, fast determination of affinities is useful during screening and development of antibodies. The kinetic parameters enable to select the most suitable antibody for the planned purpose when several monoclonals are available (Note 9). [Pg.48]

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