Diazepam-imprinted polymers

Several authors have compared imprinted polymers to biological receptors and even the term plastic antibodies has been coined to describe these remarkable materials. To a large extent, this comparison rests on a spectacular work of Mosbach and co-workers [24], who showed that theophylline- and diazepam-imprinted polymers displayed a specificity rather similar to that of polyclonal antibodies in binding studies with the template and its close structural analogues. However, it is the recognition of nucleotide or oligopeptide sequences which is... 

This section will illustrate the MIP technique for sample preparation by presenting examples of diazepam and its metabolites in hair samples.177 An anti-diazepam molecularly imprinted polymer... 

The quantitative determination of drugs by radio-immunoassay techniques using imprinted polymers instead of natural antibodies was investigated by Mosbach. Theophyllin, Diazepam and Morphine were recognised and cross-reaction tests with other but sterical similar molecules, gave similar results like the established antibody reactions [449]. Eventually the expensive antibodies can be replaced by cheap synthetic imprinted polymers [474]. 

Molecularly imprinted sorbent assays represent one of the most typical applications of biomimetic use, where imprinted polymers are used as substitutes of natural antibodies in immunoassays. The assays usually involve competitive binding of an analyte with a certain quantity of labeled ligands, in which the labeled ligand unbound is proportional to the analyte added. Because dissociation constants of common imprinted polymers are around 10 6-10 9 M, competitive binding assays could easily be performed. In practice, many molecularly imprinted sorbent assays have been developed for biologically active compounds, including theophylline, diazepam [26], S-propranolol [27], morphine, Leu-enkephalin [28], cyclosporin A [29], yohimbine [30], methyl-a-glucoside [31], corticosteroid [32], atrazine [33, 34], and 2,4-D [35]. 

X.F. Liu, F. Li, B. Yao, L. Wang, G.Y. Liu and C.Y Chai, Spectroscopic study of diazepam molecularly imprinted polymers and initiative application to conductimetric sensor based on molecularly imprinted films, Spectrosc. Spect. Anal, 30 (8) 2228-2231,2010. 

The Mosbach group described in 1993 the first assay in which polymers imprinted with either theophylline or diazepam replaced antibodies in competitive RILA... 

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