Phage selection

How do the mutations identified by phage display improve binding specificity There is as yet no direct stmctural information on the phage-selected inhibitors however they can be modeled using data from the crystal structures of other Kunitz domains bound to serine proteinases. These studies lead to the conclusion that the mutations identified by phage display improve binding specificity by maximizing complementarity between the... 

Dennis, M.S., Lazams, R.A. Kunitz domain inhibitors of tissue factor-factor Vila. II. Potent and specific inhibitors by competitive phage selection. /. Biol. 

Fig. 10. Results of phage selections of zinc finger domains for different DNA targets. The sequence of the finger-2 recognition helix for six randomly chosen clones (right) that were selected for binding to the indicated DNA target (left). Amino acid positions are in reference to the start ofthea-helix. Putative contact positions — 1,3, and 6 are boxed.

Roberts BL, Markland W, Siranosian K, Saxena, Guterman SK, Ladner RC, Protease inhibitor display M13 phage selection of high-affinity neutrophil, elastase inhibitors, Gene, 121 9-15, 1992. 

Preparations of cells for phage selection may require some procedures that can damage or modify the morphology of the final product. Treatment of tissues with proteolytic enzymes can lead to the loss of some phenotypic characteristic of the target cells. Moreover, upon isolation and culture, these cells, removed from their natural environment, could lose some phenotypic and/or structural characteristics necessary to perform a given biological activity. 

To avoid these problems, recently the possibility of using intact fragments or whole tissues as targets for phage selection has been described [40]. An... 

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