Poly polyamine graft copolymer

This paper reviews the present status of affinity separation of cells based on the biospecific interaction of cellular receptors with proteinaceous ligands immobilized on a solid-phase matrix. Special emphasis was placed on the development of new matrix materials for immuno-affinity chromatography of lymphocyte subpopulations. Our newly developed matrix of poly(2-hydroxyethyl methacrylate)/polyamine graft copolymer offered novel advantages in (1) elimination of non-specific adsorption of lymphocytes and (2) simple immobilization procedure of ligand protein through non-covalent adsorption. This matrix allowed a rapid separation of preparative quantities of pure and vital lymphocyte subpopulations (IgG-positive and -negative cells) in excellent yield. 

A series of polymine-graft copolymers of styrene [92-95] and hydroxyethyl methacrylate [96-98] were found to form a microdomain structure and exhibit unique biomedical behavior at the interface with living cells, such as blood platelets and lymphocytes. The most intensive studies were made with poly(hydroxyethyl methacryIate)-0ra/t-polyamine copolymers (HA) ... 

These suppressive effects of SA copolymers on cellular adsorption and shape change were observed for rat lymphocytes as well as platelets (58,59). Figure 2 clearly demonstrates the elimination of adsorption of lymphocytes on albuminated surfaces of SA copolymers containing 9 wt% polyamine branches (SA9). A change in the back-bone structure of the graft copolymer from polystyrene to the more hydrophilic poly(2-hydroxyethyl methacrylate) (PHEMA)... 

Graft copolymers of natural rubber and poly(methyl methacrylate) in latex form have been produced on a small scale. The rubber latex is treated with methyl methacrylate in the presence of an initiator (e.g. hydroperoxide activated by a polyamine). Generally, equal weights of rubber (dry weight) and monomer are used and the product consists of about 80% graft copolymer, 10% polyisoprene and 10% poly(methyl methacrylate). The material gives rise to vulcanizates with excellent physical properties at high hardness levels. 

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