Poly -modified electrodes oxidase

R. Garjonyte and A. Malinauskas, Glucose biosensor based on glucose oxidase immobilized in electropolymerized polypyrrole and poly(o-phe-nylenediammine) films on a Prussian blue-modified electrode, Sens. Actuators B, 63 (2000) 122-128. 

Figure 4. (left) Steady state response to 31.5 mM glucose of the ferrocene-modified poly(siloxane) / glucose oxidase / carbon paste electrodes at several applied potentials. The relay systems are indicated next to each curve, which is the mean result for four electrodes. 

Saby C, Mizutani F, Yabuki S. Glucose sensor based on carbon paste electrode incorporating poly(ethylene glycolj-modified glucose oxidase and various mediators. Analytica Chimica Acta 1995 304 33-39. 

Immobilization of Glucose Oxidase in Poly(vinyl alcohol) Modified Electrodes... 

Mizutani, R, S. Yabuki, and S. lijima (1995). Amperometric glugose-sensing electrode based on carbon paste containing poly(ethylene glycol)-modified glucose oxidase and cobalt octaethoxyphthalocyanine. Anal. Chim. Acta 300, 59-64. 

Qiu B, Lin Z, Wang J, Chen Z, Chen J, Chen G (2009) An electrochemiluminescent biosensor for glucose based on the electrochemiluminescence of luminol on the nafion/ glucose oxidase/poly(nickel(ll)tetrasulfophthalocyanine)/multi-walled carbon nanotubes modified electrode. Talanta 78(l) 76-80. doi 10.1016/j.talanta.2008.10.067... 

Lactate oxidase-modified electrodes have also been used in FIA for lactate. In particular, a platinum electrode was modified with LOD using poly-o-phenylenediamine (PPD),... 

The electropolymerization of MN4-MC catalysts on carbon supports is another possibility concerning the fabrication of modified electrodes. Qiu et al. [54] reported the development of a glucose biosensor based on poly-Ni-Pc/MWCNTs. The enzyme glucose oxidase (GOx) was also immobilized on the surface of the functionalized electrode. The sensor gready improved the emission of luminol electrochemiluminescence (ECL) in the presence of H2O2, one of the products of glucose oxidation by GOx. 

J.P. Lowry and R.D. O Neill, Partial characterization in vitro of glucose oxidase modified poly(phenylenediamine)-coated electrodes for neurochemical analysis in vivo. Electroanalysis 6, 369— 379 (1994). 

All potentials vs. screen-printed pseudo Ag/AgCl reference, except values marked with double-dagger (J), which are vs. screen-printed Ag/Pd, and 02 those marked with asterisk ( ), which are vs. Ag/3M AgCl double-junction reference electrode. Abbreviations LDH lactate dehydrogenase, LOD lactate oxidase, PVA-SbQ styrlpyridinium-modified poly(vinyl alcohol), FIA flow injection analysis. aLinear ranges using a dialysis system. 

Figure 8.12 Schematic illustration of the layer-by-layer deposition on an Au electrode initially with positively charged poly(diallydimethylammonium) chloride (PDDA) and negatively charged poly(sodium 4-styrenesulfonate). Subsequent depositions entailed (I) PDDA-modified Prussian blue nanoparticles (P-PB), followed by (II) negatively charged glucose oxidase (GOx).55 (Reprinted with permission from W. Zhao et al., Langmuir 2005, 21, 9630-9634. Copyright 2005 American Chemical Society.) (See color insert.)...

Figure 8. (right) Glucose calibration curves for the ferrocene-modified poly(ethylene oxide)/glucose oxidase/carbon paste electrodes at E =... 

Figure 6. Strip chart recorder output. Glucose calibration curve carbon electrode modified with poly(l,3-DAB)/l,r-DMF/glucose oxidase at +0.15 V vs SCE deoxygenated buffer.

Acetylcholineesterase and choline oxidase A glassy C electrode surface was modified with osmium poly (vinyl-pyridine) redox polymer containing horseradish peroxidase (Os-gel-HRP) and then coated with a co-immobilized layer of AChE and ChO. A 22 pL pre-reactor, in which ChO and catalase were immobilized on beads in series, was used to remove choline. The variation in extracellular concentration of ACh released from rat hippocampal tissue culture by electrical stimulation was observed continuously with the online biosensor combined with a microcapillary sampling probe. Measurement of ACh and Ch was carried out by using a split disc C film dual electrode. 

Karan et al. [10] reported glucose sensors using quinone modified poly-siloxane (Fig. 3.8a-A) and acrylonitrile-ethylene (Fig. 3.8a-B) co-polymers and glucose oxidase. Sensors constructed with glucose oxidase and quinone modified polysiloxane were considerably more efficient than those using acrylonitrile-ethylene system to transfer electrons from reduced glucose oxidase to a conventional carbon paste electrode. Their results coincide with those described previously for the ferrocene-modified polysiloxane system. The excellent flexibility of poly(siloxane) allows it to function as an efficient... 

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