Poly fluorescence microscopy

Fig. 43 Dry thickness of poly(2-hydroxyethyl methacrylate) (PHEMA) as a function of the position on the substrate (lower panel). The fluorescence microscopy images show the corresponding structure of fibrinogen at three positions along the PHEMA gradient (upper panel). (Reproduced with permission from [164])...

Okano et al. [84] measured changes in cytoplasmic Ca2+ concentrations in platelets adhering to HEMA-STY block copolymer (HSB) surfaces by means of fluorescence microscopy combined with a high performance image processor. Comparative studies were also carried out with the HEMA-STY random (HSR) copolymer of poly HEMA and polystyrene. Their results showed that cytoplasmic free calcium levels in platelets that were in contact with the HEMA-STY... 

The effect of temperature on fluorescence has been studied, as has the effect of salt concentration and water-soluble conjugated polymers. A method for the quantification of ssDNA dsDNA is described, as well as kinetics of mismatch hybridization and the kinetics of collision in short ss-nucleic acids. Fluorescence quenching of Cy-5 labelled oligonucleotides by poly(phenylene ethynylene) particles has been shown to be a more sensitive method than excitation of the Cy-5 fluorophore. An ultrasensitive method for the detection of DNA uses highly fluorescent conjugated nanoparticles, and detection limits below IfM were achieved. DNA transport through a carbon nanotube has also been observed using fluorescence microscopy. " ... 

Fig. 1 Fluorescence microscopy of the binding of Rh-PE-labeled paclitaxel-loaded poly(ethylene glycol)-phosphatidyl ethanolamine (PEG-PE)-based micelles to murine EL4 and large luteal cells (LLC) and human BT-20 and MCF-7 cells. (With permission from Ref. l)...

UnezaM, S. Maruyama, K. Hosoda, J.-L Nagae, 1. Koyanagi, Y Nakata, M. Ishida, O. Iwatsuru, M. Tsuchiya, S. Direct measurement of the extravasation of poly(ethylene glycol)-coated hposomes into sohd tumor tissue by in vivo fluorescence microscopy. Int. J. Pharm. 1996,144,11-17. 

Fig. 3 (a,b) Cell detachment from a poly(NiPAAm-co-DEGMA) substrate microscopy images of comeal endothelial cells after (a) standard cultivation at 37°C (1747 pm x 1385 pm) and after (b) cooling to 30°C (436 pm x 346 pm) (c,d) fluorescence microscopy images (213 pm x 162 pm, oval objects nuclei, scattered material in between fibronectin) of corneal endothelial cells on poly (NIPAAm-co-DEGMA) after (c) standard cultivation at 37°C and after (d) cooling to 30°C (reproduced from (Nitschke et al. 2007a) with permission)... 

Fluorescence microscopy was used, for instance, to gain spatially resolved information on the modification of ETFE with a sulfonated brush. The brush was prepared by free radical polymerization following the irradiation of the substrate with EUV radiation in an interference setup. Fluorescently labeled poly(allylamine) was deposited on the brush-modified surface and specifically adsorbed on sulfonated regions by electrostatic interactions between sulfonate and amino groups [6]. Observation of the surface clearly showed increased fluorescence intensity in brush-modified regions (Figure 5.5). 

The morphology can be controlled using poly(vinyl alcohol) (PVA)/ PPV precursor polymers. The morphology of fibers can be characterized by scanning electron microscopy and fluorescence microscopy. The fluorescence spectra of PVA/PPV nano fibers and of composite nano fibers made from PPV/MEH-PPV exhibit an appreciable blue shift, a stronger intensity of fluorescence, and a higher surface photovoltage in comparison to bulk material. ... 

Figure I. Fluorescence microscopy of SinorUzobium meliloti stained widi Nile red. (left) free-living cells containing multiple yellow-fluorescent intracellular inclusions of poly-P-hydroxybutyrate (right) bacteroid cells extracted from nodules of Medicago saliva with no granules.

In Table 1 we present the Zp values determined in THF and two different THE/DME mixtures. These values, on the order of 1 rm, are comparable to those reported by Discher and coworkers [38,70] and by Bates and coworkers [71,72] for PEO-PI cylindrical micelles with a core diameter of 20 nm in water. Here PEG denotes poly(ethylene oxide). Bates and coworkers deduced their values of Zp from small-angle neutron scattering experiments, whereas Discher and coworkers determined the Zp values using fluorescence microscopy. The fact that the Zp values that we determined from viscometry are comparable to those of the PEO-PI cyUndrical micelles with similar core diameters again suggests the validity of the YFY theory in treating the nanofiber viscosity data. This study demonstrates that block copolymer nanofibers have dilute solution properties similar to those of semi-flexible polymer chains. 

Journal of Applied Polymer Science 69, No.4, 25th July 1998, p.645-55 MAPPING PHASES OF POLY(VINYL ALCOHOL) AND POLY(VINYL ACETATE) BLENDS BY FTIR MICROSPECTROSCOPY AND OPTICAL FLUORESCENCE MICROSCOPY Dibbem-Brunelli D Atvars T D Z Joekes I Barbosa V C Campinas,Universidade Estadual... 

Figure 3 Example of colloidal objects observed by confocal fluorescence microscopy after mixing complementary solutions of poly(1-(4-vinylbenzyl) thymine-co-dodecyl methacrylate) P(VBT-co-DMA) and poly(9-(4-vinylbenzyl)adenine-co-dodecyl methacrylate) P(VBA-co-DMA). Reprinted with permission from Lutz, J.-F. Pfeifer, S. Chanana, M. etal. LangmuirZWiS, 22,411, Copyright 2007, American Chemical Society. ...

KEY WORDS self-healing, poly(L-lysine)-gra//-poly(ethylene glycol), methoxy-poly(ethylene glycol)-trimethylsilylether, aqueous lubrication, fluorescence microscopy... 

Fluorescence microscopy poly(L-lysine)-graft-poly (ethylene glycol)... 

An interesting novelty was reported by a German team in 2009 when they described vesicles composed of amphiphilic nanoparticles. It was demonstrated with TEM and fluorescence microscopy that CdSe/CdS core-shell nanoparticles with a brush-like coating of poly(ethylene oxide) form LUVs and GUVs. The vesicle wall is composed of a single layer of nanoparticles. Nanoparticle vesicles constitute a new class of organic-inorganic hybrid vesicles. 

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