Physcomitrella

Simple plants Physcomitrella patens Chlamydomonas reinhardtii Lemna minor Containment, clonal propagation, batch consistency, secretion of proteins into medium, regulatory compliance, homologous recombination in Physcomitrella Scalability... 

Host Plants, Systems and Expression Strategies for Molecular Farming Physcomitrella patens... 

Anterola A, Shanle E, Perroud PE, Quatrano R. (2009) Production of taxa-4(5),ll(12)-diene by transgenic Physcomitrella patens. Transgenic Res 18 655-660. 

Weise A, Altmann F, Rodriguez-Franco M, Sjoberg ER, Baumer W, Launhardt H, Kietzmann M, Gorr G. (2007) High-level expression of secreted complex glycosylated recombinant human erythropoietin in the Physcomitrella D-fuc-t D-xyl-t mutant. Plant Biotechnol J 5 389 01. 

Koprivova A, Stemmer C, Altmann F, Hoffmann A, Kopriva S, Gorr G Reski R, Decker EL. (2004) Targeted knockouts of Physcomitrella lacking plant-specific immunogenic A-glycans. Plant Biotechnol J 2 517-523. 

Physcomitrella patens (moss) Cultures gametophyte tissue sucrose and CsCl gradients Pure cpDNA other methods failed 26... 

Chalcon synthase occurs in many angiosperms, but also in early landplants, such as the moss Physcomitrella. As can be seen from Fig. 7.17a, the plant enzymes form a sister to fungal CHS, which share ancestry with polyketide... 

Petroselinum hortense PANILAIGTA Medicago sativa Arabidopsis thaliana Allium cepa Solanum lycopersicum Nicotiana tabacum Physcomitrella patens P seudomonas flucre sc. Streptcmyces griseus Prcchlcrcccccus marinus.LT.RGM... Synechcccccus sp. ALTLHG. 

Several plants A6-desaturase genes have been cloned from Anemone leveillei, borago [211], three Echium species, Marchantia polymorpha. Primula farcinosa, P. vialii and P. juliae, as well as from fungi Mortierella alpine, M. isabelina, Mucor rouxii [205] and Pythium irregulare, from the diatom Phaeodactylum tricornutum [203] and from mosses the Ceratodom purpureus and Physcomitrella patens [212], although not all of them have been expressed in plants. 

Fig. 7.1 Physcomitrella patens. Left highly homogeneous protonema tissue. Right a leafy gametophore.

During the 1990s, Physcomitrella was discovered as a new tool for functional geno-... 

Physcomitrella is a well-established plant system that, since the 1960s, has been cultivated under axenic conditions in many laboratories. It can be cultivated in liquid cultures, and such photobioreactors are scaleable. Transgenic moss strains show high genetic stability. Different biopharmaceuticals, including human growth factor, antibodies and non-glycosylated proteins such as HSA were successfully expressed and secreted into the medium. 

A. Lucumi, C. Posten, Improved in vitro culture of the moss Physcomitrella patens in a tubular pilot photobioreactor, (submitted). 

A. Baur, R. Reski, G. Gorr, Enhanced recovery of a secreted recombinant human growth factor by stabilising additives and by coexpression of human serum albumin in the moss Physcomitrella patens. Plant Biotechnol. J., in press. 

A. Baur, B. Timm, A. Weise, M. Rodriguez-Franco, G. Gorr, Genetic stability of transgenic Physcomitrella patens plants. Moss 2004, The 7th Annual Moss International Conference, Freiburg, Germany... 

There are Arabidopsis thaliana (ampl, [29]) and Physcomitrella patens (ove, [30]) mutants showing an altered cytokinin accumulation, perhaps due to changes in the biosynthetic pathway. In Arabidopsis ampl the product of the ampl gene, AMPl, is suggested to regulate the isopentenyltransferase-like enzyme and maybe also the hydrolysis of cytokinins from their conjugates. The use of plant hormone mutants in phytohormone research is discussed elsewhere in this issue. 

Fig. 8 Greenovation use a fully contained moss bioreactor. This company has established an innovative production system for human proteins. The system produces pharmacologically active proteins in a bioreactor, utilizing a moss Physcomitrella patens) cell culture system with unique properties [24]. (See Color Plate p. xxi).

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