Pythium irregulare

Fig. 2.3 HPLC analysis of ginsenosides recovered from the spent medium of Trichoderma hamatum and Pythium irregulare isolates. A ginsenoside mixture extracted from 3-year-old ginseng roots was added to the culture medium of both T. hamatum and Pythium irregulare, recovered after several days of incubation and analyzed by HPLC. The profile of ginsenosides added to the culture medium (a) included ginsenosides Rgi, Re, Rbi, Rba, Rc, Rd, and F2, as well as gypenoside XVII (G-XVII). (b-e) Profiles of ginsenosides recovered from T. hamatum isolate 3-323 (b), and Pythium irregulare isolates BR 486 (c), BR 598 (d), and BR 1068 (e).

Yousef LF, Bernards MA (2006) In vitro metabohsm of ginsenosides by the ginseng root pathogen Pythium irregulare. Phytochemistry 67 1740... 

Neculai MA et al (2009) Partial purification and characterization of three ginsenoside-metabolizing b-glucosidases from Pythium irregulare. Phytochemistry 70 1948... 

Harvey PJ et al (2001) Genetic and pathogenic variation among cereal, medic and sub-clover isolates of Pythium irregulare. Mycological Res 105 85... 

Figure 3. HPLC Analysis of Ginsenosides. Ginsenosides were isolated from spent broth in which either no organism (upper trace), Trichoderma hamatum (middle trace) or Pythium irregulare (lower trace) had been cultured for five days at 25 °C in the dark. Ginsenosides were chromatographed on a Microsorb-MV C-18 column (150 x 4.6 mm, 5 mm) using an acetonitrile H20 gradient (Nicol et al., 2002), and detected at 203 nm. The in the lower trace indicates the unknown metabolite found in the spent broth of Py. irregulare.

Braun, PG. The combination of Cylindrocarpon lucidum and Pythium irregulare as a possible cause of apple replant disease in Nova Scotia. Can J Plant Pathol 1991 13 291-297. 

Several plants A6-desaturase genes have been cloned from Anemone leveillei, borago [211], three Echium species, Marchantia polymorpha. Primula farcinosa, P. vialii and P. juliae, as well as from fungi Mortierella alpine, M. isabelina, Mucor rouxii [205] and Pythium irregulare, from the diatom Phaeodactylum tricornutum [203] and from mosses the Ceratodom purpureus and Physcomitrella patens [212], although not all of them have been expressed in plants. 

Hong H, Datla N, Reed DW, CoveUo PS, MacKenzie SL, Qiu X. (2002) High-level production of gamma-linolenic acid in Brassica juticea using a deltah desaturase from Pythium irregulare. Plant Physiol 129 354—362. 

Hong, H., Datla, N., Reed, D.W., Covello, P.S., et al.. High-level production of y-linolenic acid in Brassica juncea using a A6 desaturase from Pythium irregulare. Plant Physiol. 2002,129, 354-362. 

KOZLOWSKI, G., METRAUX, J.-P., Infection of Norway spruce Picea abies (L.) Karst.) seedlings with Pythium irregulare Buism. and Pythium ultimum Trow. histological and biochemical responses., Eur. J. Plant Pathol, 1998,104, 225-234. 

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