Photoactivatable substrates

FIGURE 3.3.2 Schematic representations of the basic concept of photoactivatable substrates. 

First Generation Photoactivatable Substrates Based on Physisorbed Blocking Agents... 

FIGURE 3.3.3 (a) The working principle of the first-generation photoactivatable substrates and (b) the projection exposure by using a fluorescence microscope. 

FIGURE 3.3.4 (a-e) Processes and (f-h) performances of cell patterning on the first-generation photoactivatable substrates. 

FIGURE 3.3.6 Applications of a first-generation photoactivatable substrate to single cell migration studies. 

FIGURE 3.3.8 Photoactivatable substrates based on various caged functional groups, (a, b) Structures and photocleavage reactions, (c) Wettability profile, (d) Cell adhesion test, COS7. 

FIGURE 3.3.9 Mechanism studies of the first-generation photoactivatable substrate impact of the type of functional groups. Cell adhesion tests to (a) bare and (b) bovine serum aibumin (BSA)-coated surfaces, (c, d) BSA adsorption quantification in various states. 

FIGURE 3.3.10 Mechanism study of the first-generation photoactivatable substrate impact of surface wettability. 

The first-generation photoactivatable substrates demonstrated a wide range of applications based on their dynamic features with high spatiotemporal resolutions. However, there was a big technical limitation regarding the cell-repelling ability of the nonirradiated surfaces. The cell-repelling ability of the nonirradiated regions does not last for more than... 

FIGURE 3.3.11 Surface design of a second-generation photoactivatable substrate and its applications, (a) Chemistry. 

FIGURE 3.3.14 A photoactivatable multiwell plate based on the third generation photoactivatable substrate. 

Fourth Generation Photoactivatable Substrates with Molecularly Defined Cell-Substrate Interactions... 

On the photoactivatable substrates discussed so far, the cells adhere to the photoirradiated regions via the interaction with proteins physically adsorbed onto the surface after photoirradiation. These proteins originally existed in the culture medium, but we are not able to specify which proteins mediate cell adhesion. This fact obscures the molecular level discussion of the signaling originated from the cell-substrate interactions. To tackle this issue, we have developed two new photoactivatable substrates, where the photoexposed surface presents molecularly controlled cell-adhesive ligand. 

HGURE 3.3.15 Mechanism study of the third generation photoactivatable substrate impact of surface charge density and PEG backfilling, (a) Experimental design, (b) Ellipsometry. (c) Zeta potential, (d) BSA adsorption, (e) Long-term persistence of cellular patterns. 

FIGURE 3.3.16 Surface design of the fourth generation photoactivatable substrate, (a) Surface and (b) molecular... 

RGURE 3.3.17 (a) Photoactivatable nanopatterned substrate based on the fourth generation photoactivatable substrate and (b) its applications. 

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