Photoactivatable substrates studies

FIGURE 3.3.6 Applications of a first-generation photoactivatable substrate to single cell migration studies. 

FIGURE 3.3.9 Mechanism studies of the first-generation photoactivatable substrate impact of the type of functional groups. Cell adhesion tests to (a) bare and (b) bovine serum aibumin (BSA)-coated surfaces, (c, d) BSA adsorption quantification in various states. 

FIGURE 3.3.10 Mechanism study of the first-generation photoactivatable substrate impact of surface wettability. 

HGURE 3.3.15 Mechanism study of the third generation photoactivatable substrate impact of surface charge density and PEG backfilling, (a) Experimental design, (b) Ellipsometry. (c) Zeta potential, (d) BSA adsorption, (e) Long-term persistence of cellular patterns. 

The p-triphosphate derivatives of ATP and AZT were synthesised and shown to inhibit DNA polymerase activity even using NRTI and NRTII resistant strains. dNTPs have been labelled at the y-phosphate by fluorophores for use in real-time PCR ° and as a method for the labelling of microtubules.Using a method known as compartmentalised selfreplication (CSR), the DNA polymerase from Pyrococcus furiosus has been evolved such that dNTPs bearing a fluorophore attached at the y-phosphate are better substrates.Ferrocene, attached via a linker as a y-phosphor-amidate has been used in an electrochemical assay to study kinase-catalysed phosphorylation reactions.The photoactivatable diazirine derivative (37) also attached to the y-phosphate was shown to be a specific label for tropomysin even in the presence of other actinomysin components. 

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