Phosphate exchange

Ooi, K., Miyai, Y., Makita, Y, and Kanoh, H., Fractionation of lithium isotopes in ion exchange chromatography with titanium phosphate exchanger, Separation Sci. Technol., 34, 1133, 1999. 

Phosphate Exchange Half-Reactions (Hydrolysis) AGw (kcal/mol)... 

McCann HG and Path EH (1958) Phosphate exchange in hydroxylapatite, enamel, dentin, and bone. J Biol Chem 231, 863-868. 

In 1947, Doudoroff, Barker and Hassid (Doudoroff et al 1947a) postulated that the reaction occurs, as shown above, by way of an intermediate compound of glucose and enzyme. They used 32P to demonstrate that inorganic phosphate exchanges with glucose-1-phosphate in the presence of the enzyme according to (9). 

Stumm W. and Leckie J.O. (1970) Phosphate exchange with sediments Its role in the productivity of surface waters. In Advances in Water Pollution Research, Vol. 2. pp. 26/1-26/16. Pergamon Press, Oxford. 

Gunnars, A., and Blomqvist, S. (1997) Phosphate exchange across the sediment-water interface when shifting from anoxic to oxic conditions an experimental comparison of freshwater and brackish-marine systems. Biogeochemistry 37, 203-226. 

Stumm, W., and Leckie, J.O. (1971) Phosphate exchange with sediments its role in the productivity of surface waters. Proceedings 5th Inti. Wat. Pollut. Res. Conf. pp. 1-16 Pergamon Press, London. 

An alternative to the two-stage end-labeling protocol involves a phosphate exchange reaction catalyzed by polynucleotide kinase. In the presence of excess amounts of ADP, polynucleotide kinase transfers a 5 -phosphoryl residue from DNA or RNA to ADP in a reversal of the phosphorylation reaction (5,12). At pH... 

Duerden C. E. (1973) Aspects of phytoplankton production and phosphate exchange in Bedford Basin, Nova Scotia. PhD Thesis, Dalhousie University, Halifax. 

Lean D. R. S. and Nawelajko C. (1976) Phosphate exchange and organic phosphorus excretion by freshwater algae. J. Fish. Board. Can. 33, 1312. 

The uiea cycle may be considered to be a mitochondrial pathway, as carbamyl phosphate synthase and ornithine transcarbamylase are mitochondrial enzymes however, the enzymes catalyzing subsequent steps of the pathway arc cytosolic-The steps leading to conversion of citrulline to ornithine occur in the cytosol. Hence, the pathway is shared by the mitochondrial and cytosolic compartments. The fumarate produced by the urea cycle is converted to malate by a cytoplasmic form of fumarase. Mittxihondrial fumarase is part of the Krebs cycle. Cytoplasmic malate can enter the mitochondrion by means of a transport system, such as the malate/phosphate exchanger or the ma ate/a-ketoglutaratc exchanger. These transport systems are membrane-bound proteins. 

ADP and [y- P]ATP at pH 7, the unlabelied phosphate exchanges with the label under enzymic catalysis. Otherwise, the termini generated on cleavage may first be dephosphorylated with alkaline phosphatase, before end-labelling. At pH 8.6 the exchange reaction is suppressed, and only the kinase reaction for determination of 5 -hydroxy termini takes place. ... 

The Schmidt group utilized a sialyl phosphite in a very different synthesis strategy (Scheme 33) [39]. Upon treatment of sialyl donor 54 with cytidine phosphoric acid 103, a phosphite-phosphate exchange reaction occmred to give com-poimd 104 exclusively as the fi isomer. Deacylation by treatment with sodium meth-oxide followed by ester saponihcation through the addition of water provided CMP-NeuAc 88. This method circmnvented the need for an oxidation step or phosphorus deprotection. This method was also applied to the synthesis of another nat-lually occmring CMP-NeuAc derivative 105 [40]. 

It should be noted that the [32P]phosphate content of a phosphoprotein does not necessarily correspond to its total phosphate content. Usually, endogenous protein-bound phosphate exchanges with the [32p]-phosphate in the medium during a prolonged incubation. In case of doubt, both the [32]phosphate and the... 

Sediment samples on the other hand may be treated in different ways, depending on the information required. Water contained in sediments can be removed and analyzed by the same methods as for aqueous samples, but speciation information on the solid fraction is more difficult to acquire. Series of sequential extractions are already employed to acquire the information needed to understand the cycling of As in sediments, for example, on water-soluble, phosphate-exchangeable, organically-bound and residual phases in such media." Literature on extraction procedures for detection, hyphenated by chromatographic techniques, is much more recent extractions of... 

SLC37 Sugar-phosphate/phosphate exchanger 4 Glycogen storage disease non-la... 

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