Phage cDNA display

Crameri R, Kodzius R Xhe powerful combination of phage surface display of cDNA libraries and high throughput screening. Comb Chem High Xhroughput Screen 2001 4 145-155. 

Cochrane, D., Webster, C., Masih, G., and McCafferty, J. (2000). Identification of natural ligands for SH2 domains from a phage display cDNA library. J. Mol. Biol. 297, 89-97. 

Crameri, R., Jaussi, R., Menz, G., and Blaser, K. (1994). Display of expression products of cDNA libraries on phage surfaces. Eur. J. Biochem. 226, 53-58. 

Crameri, R., and Suter, M. (1993). Display of biologically active proteins on the surface of filamentous phages a cDNA cloning system for selection of functional gene products linked to the genetic information responsible for their production. Gene 137, 69-75. 

Sche, P. P., McKenzie, K. M., White, J. D., and Austin, D. J. (1999). Display cloning functional identification of natural product receptors using cDNA-phage display. Chem. Biol. 6, 707-716. 

More recently, Janda has described the production of a galactopyranosidase antibody in response to hapten [96]. This was designed to accommodate several features of the transition state for glycoside hydrolysis notably a flattened half-chair conformation and substantial sp2 character at the anomeric position. Some 100 clones were isolated in response to immunization with [96] and used to generate a cDNA library for display on the surface of phage (Appendix entry 7.3) (Janda et al., 1997). Rather than proceed to the normal screening for turnover, Janda then created a suicide substrate system to trap the catalytic species. 

Peptide libraries Intermediaries for non peptide drugs Mirror image phage display Peptides with effector functions Select binders on unknown antigens From 2D gels From expression cDNA libraries... 

To circumvent some of the limitations of direct immunization, phage display technology has been applied to the preparation of fully human MABs. Gene libraries of cDNA from nonimmune or immunized donor lymphocytes are expressed in bacteriophages. The bacteriophages display functional antibody fragments and can... 

Danner, S. and Belasco, J. G. (2001) T7 phage display A novel genetic selection system for cloning RNA-binding protein from cDNA libraries. Proc. Natl. Acad. Sci. USA 98, 12,954-12,959. 

Pereboeva, L. A., Pereboev, A. V., Wang, L. F., and Morris, G. E. (2000) Hepatitis C epitopes from phage-displayed cDNA libraries and their assembly into improved chimeric antigens. J. Med. Virol. 60, 144-151. 

More recent developments using cDNA libraries of B lymphocytes and phage display technology have made it possible to produce complete human mAbs. The... 

To identify relevant IgE binding components from natural source extracts, various techniques like IgE immunoscreening of expression cDNA libraries, reverse transcriptase polymerase chain reaction (PCR), or phage-display technology combined with immunoscreening are used (Wallner et al. 2004). 

Crameri R, Elemmann S, Blaser K, pJuFo A phagemid for display of cDNA libraries on phage surface suitable for selective isolation of clones expressing allergens, Adv. Exp. Med. Biol., 409 103-110, 1996. 

Light J, Maki R, Assa-Munt N, Expression cloning of cDNA by phage display selection, Nucleic. Acids. Res., 21 4367 4368, 1996. 

Display on lambda phages has been described with two major coat proteins the gpV tail protein [23, 24] and the gpD head protein [25, 26]. Both proteins accept C-terminal fusions, which offers alternative systems for displaying cDNA libraries. A high level of display is possible as demonstrated by the total modification of gpV [27] and gpD [25] proteins. The structure of gpD has been solved recently [28] this should help the design of optimal display vectors. 

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