Bacteriophage display

Figure 5.8. Schematic illustration of T7 bacteriophage display system. The gene encoding the protein of interest is fused to the gene 10 protein that encodes a coat protein in the head of the phage. Cloning is to the C-terminus of coat protein 10. Figure adapted from Sche et al. (1999).

Lowman, H. (1997). Bacteriophage display and discovery of peptide leads for drug development. Ann. Rev. Biophys. Biomol. Struct. 26, 401-424. 

To circumvent some of the limitations of direct immunization, phage display technology has been applied to the preparation of fully human MABs. Gene libraries of cDNA from nonimmune or immunized donor lymphocytes are expressed in bacteriophages. The bacteriophages display functional antibody fragments and can... 

A number of methods for the selection of bacteriophage displaying V gene products by affinity purification have been described including ... 

Abrol S, Sampath A, Arora K, Chaudhary VK, Construction and characterization of Ml3 bacteriophages displaying gpl20 binding domains of human CD4, Indian J. Biochem. Biophys., 31 302-309, 1994. 

Smith GP, Schultz DA, Ladbury JE, A ribonuclease S-peptide antagonist discovered with a bacteriophage display library, Gene, 128 37 42, 1993. 

In Vivo Bacteriophage Display for the Discovery of Novel Peptide-Based Tumor-Targeting Agents... 

A Kushwaha, PS Chowdhury, K Arora, S Abrol, VK Chaudhary. Construction and characterization of Ml3 bacteriophages displaying functional IgG-binding domains of staphylococcal protein A. Gene 151 45-51, 1994. 

McLafferty MA, Kent RB, Ladner RC, Markland W (1993) Ml 3 bacteriophage displaying disulfide-constrained microproteins. Gene 128(l) 29-36... 

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