Phages filamentous

Crameri, R., and Suter, M. (1993). Display of biologically active proteins on the surface of filamentous phages a cDNA cloning system for selection of functional gene products linked to the genetic information responsible for their production. Gene 137, 69-75. 

Riechmann, L., and Holliger, P. (1997). The C-terminal domain ofTolA is the coreceptor for filamentous phage infection ofE. coli. Cell 90, 351-360. 

Many aspects of DNA replication in filamentous phages are similar to that of < >X 174. The unique property, release without cell killing, can be briefly discussed. The release from the cell occurs by a budding process in which the virus particle is always released from the cell with the end containing the A protein first. Interestingly, the orientation of the virus particle across the cell membrane is the same for its entry and exit from the cell. There is no accumulation of intracellular virus particles the assembly of mature virus particles occurs on the inner cell membrane and virus assembly is coupled with the budding process. 

Smith GP, Scott JK. Libraries of peptides and proteins displayed on filamentous phage. Methods Enzymol 1993 217 228-257. 

Another viral chromosome that can be used as a vector is that of the filamentous phage Ml3. The M13 chromosome is a single-stranded DNA molecule which when inserted into the bacterial host replicates outside the bacterial chromosome in the cytoplasm. The virus is then reassembled and released from the bacterial cell without cell lysis. 

Hart SL, Knight AM, Harbottle RP, et al. Cell binding and intemaUzation by filamentous phage displaying a cyclic Arg-Gly-Asp-containing peptide. J Biol Chem 1994 269(17) 12468-12474. 

Figure 14.9 Filamentous phage, M13 or fd, displaying a protein fused to a gene III protein.

Infection of E coli with filamentous phage is a chronic one in that the phage does not lyse the E. coli. It is therefore possible to maintain filamentous phage either as a phage-secreting E coli strain or as a suspension of bacteriophage particles... 

Development of Filamentous Phage Vectors for Antibody Display... 

Hoogenboom, H. R., Griffiths, A. D., Johnson, K. S., Chiswell, D. J., Hudson, P., and Winter, G (1991) Multi-subunit proteins on the surface of filamentous phage, methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Res 19,4133-4137... 

McCafferty, J., Griffiths, A D, Winter, G., and Chiswell, D. J. (1990) Phage antibodies-filamentous phage displaying antibody variable domains. Nature 348, 552-554... 

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