Peptide synthesizers, multiple Synthesizer

The synthesis can be carried out manually or automated using a Symphony/Multiplex multiple peptide synthesizer or an Argonaut Nautilus. 

Instrument Multiple peptide synthesizer SYRO (MultiSynTec) equipped with a 48-reaction vessel block (see Note 1). 

Besides performing multiple syntheses in parallel, the accelerating of reaction rates may also achieve the same result— production of multiple peptides in the same time period. CEM (http //www.cem.com) has employed microwave irradiation to shorten both coupling and deprotection times. Their synthesizer, Odyssey (Fig. 7), achieves one cycle of peptide synthesis (Fmoc-based) in less than 10 min and is theoretically capable of the synthesis of 12 peptides in a row. However, for each new peptide the reaction vessel has to be cleaned and new batch of resin has to be transferred into it. This step raises concerns with the authors of this review about the possibility of cross-contamination. 

Centrifugation is a powerful technique allowing the parallel processing of an unlimited number of reaction compartments (101). The first centrifugal multiple peptide synthesizer, Compas 242 (76,102), utilized centrifugation for liquid removal from the functionalized cotton used as the solid support or from resin contained in polypropylene mesh bags (703). This system enabled the automation of tea-bag synthetic methodology. In principle, however, separation of solid and liquid phases was still accomplished by filtration. 

Lebl, M., Stierandova, A., Eichler, J., et al. (1992) An automated multiple solid phase peptide synthesizer utilizing cotton as a carrier. In Innovation and Perspectives in Solid Phase Peptide Synthesis (Epton, R., ed.). Intercept Limited, Andover, UK, pp. 251-257. 

Zuckermann, R. N., Siani, M. A., and BanvUle, S. C. (1992) Control of the zymate robot with an external computer Construction of a multiple peptide synthesizer. Lab. Robotics Automation 4, 183-192. 

Synthetic library Iterative Many peptides can be synthesized and Peptide mixture requires multiple... 

The main advantages of the OBOC method include 1) A large number (e.g., 10 —10 ) of peptides can be synthesized (Fig. lb) and screened rapidly and simultaneously by using either one or a combination of both on-bead and solution-phase assays, and 2) multiple peptide ligands with completely different motifs often can be identified in a single screening. This occm-rence is the opposite of the (convergent) iterative approach (see... 

In 1998, Zinsser introduced the PepSy, their next generation multiple peptide synthesizer, which is capable of synthesizing 32 or 96 peptides in reaction vessels of 3.5 and 1.8 mL, respectively. The reaction block can be vortexed for mixing of the resin and the reagents and solvents are distributed with four specially designed probes. The filtration of the resin is from the top. 

In 1989, Advanced ChemTech (ACT) introduced the Model 350 Simultaneous Multiple Peptide Synthesizer capable of assembling up to 96 peptides at scales from 5 to 50 pmol using the Fmoc method. Two different sized reaction vessels are available 600 pL and 1.5 mL. The first Model 350 was originally designed similar to the SMPS 350 by Zinsser, however during the next several years, ACT added additional features, such as an orbital shaker for resin agitation to differentiate the product. ... 

ACT produces the Model 348 Multiple Peptide Synthesizer for researchers who do not require a sophisticated instrument for multiple peptide synthesis. This synthesizer is similar to the Model 396 MBS except it possesses a single robotic arm. 

Most synthetic methods for the generation of peptide libraries have been derived from various multiple parallel peptide synthesis techniques developed since 1984.bs-i l Consequently, the sohd supports used for hbrary synthesis are essentially the same as those used for multiple peptide synthesis. Standard divinylbenzene cross-linked polystyrene resins are typically used for hbraries that are cleaved from the resin and screened in solution.P Polyoxyethylene-grafted polystyrene resins,f l or acrylamide-polyoxyethylene copolymers, P on the other hand, are the sohd supports of choice for the synthesis of resin-bound peptide hbraries screened in sohd-phase binding assays.P Such resins are compatible with both organic solvents used for peptide synthesis, as well as aqueous buffers used in the bioassays. Various segmental supports previously employed for multiple peptide syntheses have also been utilized for the synthesis of peptide libraries, including polypropylene pins, PI cotton,t cellulose membrane,and glass shdes.P ... 

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