Pepsin, applications

Berry, A., Dodge, T.C., Pepsin, M. and Weyler, W. (2002) Application of metabolic engineering to improve both the production and use of biotech indigo. Journal of Industrial Microbiology Biotechnology, 28, 127—133. 

Similarly, lysine has been incorporated into gluten hydrolyzate and lysine, threonine and tryptophan have been individually incorporated into zein hydrolyzates. Lysine, methionine, and tryptophan were incorporated simultaneously into hydrolyzates of protein from photosynthetic origin. A very interesting application of this procedure involved the preparation of low-phenylalanine plasteins from a combination of fish protein concentrate and soy protein isolate by a partial hydrolysis with pepsin then pronase to liberate mainly phenylalamine, tyrosine, and tryptophan, which were then removed on sephadex G-15. Desired amounts of tyrosine and tryptophan were added back in the form of ethyl esters and a plastein suitable for feeding to infants afflicted with phenylketonuria was produced. 

Pepsin [9001-75-6] - [AMINOACIDS - SURVEY] (Vol 2) -detection of [BIOPOLYMERS - ANALYTICAL TECHNIQUES] (Vol 4) -screening for (BIOPOLYMERS - ANALYTICAL TECHNIQUES] (Vol 4) -in therapeutic applications [ENZYME APPLICATIONS - THERAPEUTIC] (Vol 9) -use in blood fractionation [FRACTIONATION, BLOOD - PLASMA FRACTIONATION] (Vol 11)... 

Mucus turnover rate. The turnover rate of the mucus layer is another physiological factor that affects mucoadhesion. Mucus turnover limits the potential duration of adhesion at the desired site of application. Within the GI tract, mucus is lost continuously secondary to enzymatic degradation (pepsin, lysosomal enzymes, and pancreatic enzymes), acid... 

Pepsin Obtained from the glandular layer of hog stomach. Produced as a white to light tan, water-soluble powder amber paste or clear, amber to brown, aqueous liquids. Major active principle pepsin. Typical applications used in the preparation of fishmeal and other protein hydrolysates and in the clotting of milk in manufacture of cheese (in combination with rennet). 

Rennet, Bovine Aqueous extracts made from the fourth stomach of bo vines. Produced as a clear, amber to dark brown liquid or a white to tan powder. Major active principle protease (pepsin). Typical application used in the manufacture of... 

Solutions containing active enzymatic proteins (protease, lipase, trypsin, pepsin, prophase, or cellulase) or their mixtures, adjusted to the nature of the matrix of the solid material of biological origin [79, 83, 84]. The aim of the procedure is to break up proteins, polysaccharides, or fat chains and release the constituent amino acids, sugars, or short aliphatic chains. Enzymatic decomposition of the matrix can be considerably enhanced by application of ultrasound the process can, for example, increase the efficiency of disintegration of cell walls in yeast and thus improve the recovery of selenium by as much as 20 % [85]. 

Medicinal Uses. One of the earliest medicinal applications of the thiol proteases was the use of fig latex as an anthelmintic agent 16, 17). In more recent times papain has been used as a digestive aid in the treatment of dyspepsia and gastric distress. Its usefulness in this respect derives from the fact that papain remains active under acid conditions and is resistant to attack by pepsin. The intraperitoneal injection of sterile solutions of papain has proved to be effective in preventing post-operative adhesions. Papain is sometimes referred to as a T io-logical scalpel because of its specific proteolytic action on dead tissue without affecting live tissue. For this reason it serves as a very useful debridement in the treatment of bums and the removal of scar tissue. When used in combination with antibiotics, it has proved to be effective for topical treatment of ulcerating and infected lesions. 

Three main protein peaks were found localized in the areas corresponding to (1) pepsin, (2) mucosubstances M2 and M3, in which albumin was also located, and (3) M4-X1, where y-globulin was present. Only one major carbohydrate area was found which coincided with that traced from the PAS-stained zone of the electropherogram, corresponding to the area of mucosubstance M3. The latter, on Gaussian curve analysis, showed the presence of many components, however, which extended from the application point to a point 3.5 cm from the anode. 

The localization of the pepsin peak in paper electropherograms in acid gastric juice is not difficult. With our technique (C ) the pepsin band in native or dialyzed and lyophilized acid gastric juice travels on paper 5-5.5 cm from the application point within 5 hours, whereas the... 

Ricin (castor bean) immunotoxin has been developed to attack the CD5 T-cell antigen (present in T-cell and some B-cell malignancies) as well as the interleukin-2 receptor of cancerous tumors. Ne-urobiological applications of ricin involve the study of brain function via lesioning. It is also used as a reagent for pepsin and trypsin, and as a commercial mole killer. Historically, it has been used as a biochemical warfare agent. 

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