PEND protein

PEND protein DNA-binding protein in the inner envelope membrane of the developing chloroplast... 

Parthasarathy, R. and Lee, S. C. Site-specific ligation of proteins to synthetic particles. US Patent application, pending (1999). 

Arenkov et al. prepared poly(acrylamide) gel pads for use in protein microarrays [199], The gels were prepared by photopolymerization of acrylamide and crosslinkers. Capture probes were immobilized, either by use of glutaraldehyde or by converting some of the acrylamide groups into hydrazides and subsequent coupling of aldehyde-modified antibodies to the pending hydrazide groups. Then, immunoassays were performed on the pads, either assays with directly labeled analytes or sandwich assays. Furthermore, the gel pads were used for enzyme activity studies. 

Tt is well recognized that the nutritional value of dietary proteins de-pends primarily on the content of their constituent amino acids, especially of their essential amino acids. Because of deficiencies of lysine and methionine, and to a lesser extent of a few other amino acids, proteins from plants and other alternative sources have low biological quality. Moreover, incomplete digestion of the protein also may result in a lack of complete availability (I) of the essential amino acids and may further reduce its value. In many raw plant foodstuffs such as soybeans, common beans, or unprocessed protein foods, undenatured... 

Labs Lab tests revealed a WBC count of 22,000/ xL (98% PMNs) and Cr 2.0 mg/dL. Results of lumbar puncture showed the following WBC count 4000/ j,L, protein 120 mg/dL, and glucose 35 mg/dL. Results of studies on blood, OF, sputum cultures, and Gram stains are pending. AL has NKDA. 

Table 20-3 lists the principal plasma proteins and their half-lives, pi, molecular weights, and preferred method of analysis the individual proteins are listed in the order of their electrophoretic mobilities in agarose gels at pH 8.6. These proteins are described later in this chapter other chapters in this book describe many more proteins enzymes (see Chapter 21) lipoproteins (see Chapter 26) hormones (see Chapter 28) and hemoglobin, fibrinogen, and other coagulation proteins (see Chapter 31). The interim consensus reference intervals for 14 plasma proteins are listed in Table 20-4, pending the publication of more definitive intervals. . 

Ultracentrifugal studies as to the homogeneity of this fraction were unsatisfactory because of the limitations of materials. No final statement can, therefore, be made concerning the molar proportions of zinc to protein, pending establishment of the molecular weight. However, the minimum molecular weight based on zinc content would be 21,700. The magnitude of the zinc content is comparable to that encountered in other metallopro-teins (Tables I, II). The isoelectric point was found close to pH 6.5. 

Analytical and enz5onological data lead to the conclusion that zinc is a structural and functional component of ADH, and that it participates in the mechanism of its enzymatic action (Vallee and Hoch, 1955b). The four metal atoms are firmly bound to the protein apoenzyme. Pending the results of investigation in progress, this discussion will assume that the four zinc atoms are bound to the protein in an equivalent manner, and that each atom of zinc acts independently of the other three in the catalytic action of alcohol dehydrogenase. This seems the more acceptable view at present, since Hayes and Velick (1954) found the four binding sites of DPN to be equivalent. 

The ability to clone and express commercially useful quantities of recombinant human proteins in bacterial, insect cell, yeast fermentation systems, or transgenic animals has enabled the development and introduction into the marketplace of otherwise unavailable lifesaving protein drugs. Numerous recombinant human protein and biotechnology products are in clinical trials or pending regulatory agency approval. 

IgG is a 160 kDa protein that is administered intravenonsly or intramuscularly. The concentration of IgG for most intravenous formulations is 10%, while it can go up to 18% for intramuscular formulations. The sonrce material for IgG in most production processes is Cohn fractions II + III [16,17], The paste is generally collected by centrifugation and sns-pended in water for injection. The solution is then clarified... 

---