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Pathway engineering construction

In this chapter, 1 describe enzymes converting cephalosporin C to 7-ACA, and the construction of a novel strain for the direct production of 7-ACA and 7-ADACA by metabolic pathway engineering (6,7). [Pg.733]

Morrone D, Lowry L, Determan MK, Hershey DM, Xu M, Peters RJ (2010) Increasing diterpene yield with a modular metabolic engineering system in E. coli comparison of MEV and MEP isoprenoid preciusor pathway engineering. Appl Microbiol Biotechnol 85 1893-1906 Na D, Kim TY, Lee SY (2010) Construction and optimization of synthetic pathways in metabolic engineering. Curr Opin Microbiol 13 363-370... [Pg.112]

Steinbuchel, A. and Lutke-Eversloh, T. 2003. Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms. Biochemical Engineering Journal 16 81-96. [Pg.39]

Further work at EniTecnologies was conducted with Rhodococcus strains. Rhodococ-cus was selected for its metabolical versatility, easy availability in soils and water, and remarkable solvent tolerance. Its capabilities for catalyzing diverse transformation reactions of crude oils, such as sulfur removal, alkanes and aromatics oxidation and catabolism caught their attention. Hence, genetic tools for the engineering of Rhodococcus strains have been applied to improve its biotransformation performance and its tolerance to certain common contaminants of the crude oil, such as cadmium. The development of active biomolecules led to the isolation and characterization of plasmid vectors and promoters. Strains have been constructed in which the careful over-expression of selected components of the desulfurization pathway leads to the enhancement of the sulfur removal activity in model systems. Rhodococcus, Gordona, and Nocardia were transformed in this way trying to improve their catalytic performance in BDS. In a... [Pg.283]

Despite these field trials, there has never been a release of a geneticaly engineered microorganism for bioremediation purposes. One should therefore bear in mind, when considering pathway construction as a route to bioremediation, that the constructed organism cannot carry out its programmed function without interacting with its environment. That we can carry out fairly complex alterations of the... [Pg.375]

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See also in sourсe #XX -- [ Pg.306 ]



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