5- P. putida strains

Naphthalene dioxygenase from P. putida strain FI is able to oxidize a number of haloge-nated ethenes, propenes, and butenes, and d5 -hept-2-ene and cis-oct-2-ene (Lange and Wackett 1997). Alkenes with halogen and methyl substituents at double bonds form allyl alcohols, whereas those with only alkyl or chloromethyl groups form diols. 

Althongh the prodnct from the transformation of toluene by mntants of Pseudomonas putida lacking dehydrogenase activity is the cis-2R,3S dihydrodiol, the cis-2S,3R dihydrodiol has been synthesized from 4-iodotoluene by a combination of microbiological and chemical reactions. P. putida strain UV4 was used to prepare both enantiomers of the di-dihydrodiol, and iodine was chemically removed nsing H2 -Pd/C. Incubation of the mixtnre of enantiomers with P. putida NCIMB 8859 selectively degraded the 2R,3S componnd to prodnce toluene cis-2S,3R dihydrodiol (Allen et al. 1995). 

Mutants impaired in the synthesis and accumulation of PHAs have been obtained from conventional chemical mutagenesis or transposon mutagenesis from many bacteria. Mutant PHB4 from R. eutropha strain H16 [130] and mutant GpP104 from P. putida strain KT2442 [36] are most probably those mutants which were mostly used, although the genotypes of these mutants were never analyzed. 

Pseudomonas stutzeri, P. alcaligenes, and P. putida strains were isolated and proven to remove DBT, benzothiophene, thioxanthene, and thianthrene by decomposition into water-soluble substances [120],... 

In Section 23.1 of this Chapter, the method for obtaining organic solvent-resistant microorganisms was described. This invention was awarded to AIST [103] and was shared with Tonen Corp. The microorganisms capable of desulfurization were mutated without jeopardizing the BDS catalytic properties leading to development of three P. putida strains reported in Section 23.1. 

Trichloroethene and aromatic compounds — A striking example is the degradation of trichloroethene by different strains of Pseudomonas sp. grown with phenol (Folsom et al. 1990) or with toluene. This capability has already been noted in Section 4.4.1.1 in the context of monooxygenase reactions, and has attracted attention for the bioremediation of contaminated sites (Hopkins and McCarty 1995). Conversely, toluene degradation is induced (a) by trichloroethene in a strain of P. putida (Heald and Jenkins 1994) and (b) in P. mendocina — although not in Burkholderia (Pseudomonas) cepacia or P. putida strain FI — by trichloroethene, pentane, and... 

In P. putida strain FI that degrades toluene with the methyl group intact (Finette et al. 1984) ... 

For the nah operon in P. putida strain G1064 involved in degrading naphthalene to salicylate (Eaton and Chapman 1992 ), the enzymes are generally induced by growth with salicylate (Austen and Dunn 1980) ... 

The naphthalene dihydrodiol dehydrogenase NahB from P. putida strain G7 has been purified as the his-tagged enzyme, and shown to catalyze also the dehydrogenation of biphenyl-2,3-dihydrodiol. biphenyl-3,4-dihydrodiol, and 2,2, 5,5 -tetrachlorobiphenyl-3,4-dihydrodiol (Barriault et al. 1998). In addition, 1,2-dihydroxynaph-thalene dioxygenase carried out extradiol fission of 3,4-dihydrox-ybiphenyl at both the 2,3- and 4,5-positions. 

Naphthalene, anthracene, and phenanthrene by P. putida strain GZ44 (Goyal and Zylstra 1996) ... 

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